Detailed information of TA system TA027839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1251200..1252117
• Replicon: chromosome
• Accession: NC_017912
• Organism: Bacillus amyloliquefaciens Y2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: MUS_RS06450
• Protein ID: WP_007407256.1
• Coordinates: 1251371..1252117 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2C3Z8
• Locus tag: MUS_RS06445
• Protein ID: WP_014417527.1
• Coordinates: 1251200..1251370 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUS_RS06400	1246406..1246918	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
MUS_RS21600	1247031..1247690	+	660	WP_014417523.1	terminase	-
MUS_RS06410	1247588..1248055	+	468	Protein_1218	hypothetical protein	-
MUS_RS06415	1248068..1248439	+	372	WP_014417525.1	hypothetical protein	-
MUS_RS06420	1248444..1248641	+	198	WP_007610833.1	XkdX family protein	-
MUS_RS06425	1248698..1249459	+	762	WP_014417526.1	hypothetical protein	-
MUS_RS06430	1249511..1249774	+	264	WP_003154815.1	hemolysin XhlA family protein	-
MUS_RS06435	1249788..1250051	+	264	WP_003154813.1	phage holin	-
MUS_RS06440	1250065..1250943	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
MUS_RS06445	1251200..1251370	-	171	WP_014417527.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
MUS_RS06450	1251371..1252117	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MUS_RS06455	1252221..1253219	-	999	WP_003154805.1	inorganic phosphate transporter	-
MUS_RS06460	1253232..1253849	-	618	WP_003154804.1	DUF47 domain-containing protein	-
MUS_RS06465	1254135..1255451	-	1317	WP_007610842.1	amino acid permease	-
MUS_RS06470	1255773..1256723	+	951	WP_014417529.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1237347..1309830	72483

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T27839 WP_007407256.1 NC_017912:c1252117-1251371 [Bacillus amyloliquefaciens Y2]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T27839 NC_017912:c1252117-1251371 [Bacillus amyloliquefaciens Y2]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.78 Da
• Isoelectric Point: 10.9951

>AT27839 WP_014417527.1 NC_017912:c1251370-1251200 [Bacillus amyloliquefaciens Y2]
MEQAFQNGQPEKRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT27839 NC_017912:c1251370-1251200 [Bacillus amyloliquefaciens Y2]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAAAAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2C3Z8