Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3812172..3812697 Replicon chromosome
Accession NZ_CP123008
Organism Escherichia coli strain NWU_4

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag QEN27_RS18295 Protein ID WP_029305600.1
Coordinates 3812392..3812697 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag QEN27_RS18290 Protein ID WP_000813634.1
Coordinates 3812172..3812390 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEN27_RS18275 (3807328) 3807328..3808227 + 900 WP_000963206.1 nucleotide-binding protein -
QEN27_RS18280 (3808277) 3808277..3810502 - 2226 WP_040073691.1 P-loop NTPase fold protein -
QEN27_RS18285 (3810504) 3810504..3811592 - 1089 WP_000952217.1 transcriptional repressor PifC -
QEN27_RS18290 (3812172) 3812172..3812390 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
QEN27_RS18295 (3812392) 3812392..3812697 + 306 WP_029305600.1 type II toxin-antitoxin system toxin CcdB Toxin
QEN27_RS18300 (3812698) 3812698..3813279 + 582 Protein_3599 tyrosine-type recombinase/integrase -
QEN27_RS18305 (3813255) 3813255..3813395 + 141 Protein_3600 RepB family plasmid replication initiator protein -
QEN27_RS18310 (3813983) 3813983..3815149 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -
QEN27_RS18315 (3815149) 3815149..3816120 + 972 WP_000817031.1 ParB/RepB/Spo0J family plasmid partition protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3786297..3816120 29823


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11716.55 Da        Isoelectric Point: 6.4674

>T278373 WP_029305600.1 NZ_CP123008:3812392-3812697 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVPVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT278373 WP_000813634.1 NZ_CP123008:3812172-3812390 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References