Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3144097..3144594 Replicon chromosome
Accession NZ_CP123008
Organism Escherichia coli strain NWU_4

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QEN27_RS15280 Protein ID WP_001173343.1
Coordinates 3144421..3144594 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QEN27_RS15275 Protein ID WP_000398619.1
Coordinates 3144097..3144393 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEN27_RS15255 (3140828) 3140828..3141547 - 720 WP_000611288.1 two-component system response regulator DcuR -
QEN27_RS15260 (3141544) 3141544..3143175 - 1632 WP_001216461.1 sensor histidine kinase -
QEN27_RS15265 (3143356) 3143356..3143586 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
QEN27_RS15270 (3143598) 3143598..3143870 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
QEN27_RS15275 (3144097) 3144097..3144393 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QEN27_RS15280 (3144421) 3144421..3144594 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QEN27_RS15285 (3144713) 3144713..3146230 - 1518 WP_001295074.1 lysine--tRNA ligase -
QEN27_RS15290 (3146467) 3146467..3147924 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T278372 WP_001173343.1 NZ_CP123008:3144421-3144594 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT278372 WP_000398619.1 NZ_CP123008:3144097-3144393 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References