Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2548688..2549283 Replicon chromosome
Accession NZ_CP123008
Organism Escherichia coli strain NWU_4

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag QEN27_RS12460 Protein ID WP_000239579.1
Coordinates 2548688..2549038 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag QEN27_RS12465 Protein ID WP_001223208.1
Coordinates 2549032..2549283 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEN27_RS12440 (2543965) 2543965..2544987 - 1023 WP_001339490.1 ABC transporter permease -
QEN27_RS12445 (2545001) 2545001..2546503 - 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
QEN27_RS12450 (2546813) 2546813..2547769 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
QEN27_RS12455 (2548079) 2548079..2548609 + 531 WP_000055072.1 inorganic diphosphatase -
QEN27_RS12460 (2548688) 2548688..2549038 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
QEN27_RS12465 (2549032) 2549032..2549283 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
QEN27_RS12470 (2549496) 2549496..2549837 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
QEN27_RS12475 (2549840) 2549840..2553619 - 3780 WP_000060901.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T278369 WP_000239579.1 NZ_CP123008:c2549038-2548688 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT278369 WP_001223208.1 NZ_CP123008:c2549283-2549032 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References