Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2064357..2065011 Replicon chromosome
Accession NZ_CP123008
Organism Escherichia coli strain NWU_4

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag QEN27_RS10135 Protein ID WP_000244772.1
Coordinates 2064357..2064764 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QEN27_RS10140 Protein ID WP_000354046.1
Coordinates 2064745..2065011 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEN27_RS10115 (2060314) 2060314..2062047 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
QEN27_RS10120 (2062053) 2062053..2062763 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QEN27_RS10125 (2062788) 2062788..2063684 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QEN27_RS10130 (2063796) 2063796..2064317 + 522 WP_001055874.1 flavodoxin FldB -
QEN27_RS10135 (2064357) 2064357..2064764 - 408 WP_000244772.1 protein YgfX Toxin
QEN27_RS10140 (2064745) 2064745..2065011 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QEN27_RS10145 (2065254) 2065254..2066234 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
QEN27_RS10150 (2066311) 2066311..2066970 - 660 WP_000250274.1 hemolysin III family protein -
QEN27_RS10155 (2067134) 2067134..2067445 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
QEN27_RS10160 (2067490) 2067490..2068923 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
QEN27_RS10165 (2068980) 2068980..2069723 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T278368 WP_000244772.1 NZ_CP123008:c2064764-2064357 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT278368 WP_000354046.1 NZ_CP123008:c2065011-2064745 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References