Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1928094..1928677 Replicon chromosome
Accession NZ_CP123008
Organism Escherichia coli strain NWU_4

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag QEN27_RS09520 Protein ID WP_000254745.1
Coordinates 1928094..1928429 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag QEN27_RS09525 Protein ID WP_000581937.1
Coordinates 1928429..1928677 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEN27_RS09500 (1923563) 1923563..1923925 + 363 WP_000034929.1 hypothetical protein -
QEN27_RS09505 (1923981) 1923981..1925279 - 1299 WP_000036723.1 phosphopyruvate hydratase -
QEN27_RS09510 (1925367) 1925367..1927004 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
QEN27_RS09515 (1927232) 1927232..1928023 - 792 WP_136721162.1 nucleoside triphosphate pyrophosphohydrolase -
QEN27_RS09520 (1928094) 1928094..1928429 - 336 WP_000254745.1 endoribonuclease MazF Toxin
QEN27_RS09525 (1928429) 1928429..1928677 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
QEN27_RS09530 (1928755) 1928755..1930989 - 2235 WP_000226815.1 GTP pyrophosphokinase -
QEN27_RS09535 (1931037) 1931037..1932338 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T278367 WP_000254745.1 NZ_CP123008:c1928429-1928094 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT278367 WP_000581937.1 NZ_CP123008:c1928677-1928429 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References