Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1602769..1603606 Replicon chromosome
Accession NZ_CP123008
Organism Escherichia coli strain NWU_4

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag QEN27_RS07965 Protein ID WP_000227784.1
Coordinates 1603064..1603606 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag QEN27_RS07960 Protein ID WP_001297137.1
Coordinates 1602769..1603080 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEN27_RS07935 (1597789) 1597789..1598736 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
QEN27_RS07940 (1598758) 1598758..1600749 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
QEN27_RS07945 (1600739) 1600739..1601353 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
QEN27_RS07950 (1601353) 1601353..1601682 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
QEN27_RS07955 (1601694) 1601694..1602584 + 891 WP_000971336.1 heme o synthase -
QEN27_RS07960 (1602769) 1602769..1603080 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
QEN27_RS07965 (1603064) 1603064..1603606 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
QEN27_RS07970 (1603662) 1603662..1604597 - 936 WP_001297127.1 tetratricopeptide repeat protein -
QEN27_RS07975 (1605005) 1605005..1606369 + 1365 WP_001000978.1 MFS transporter -
QEN27_RS07980 (1606497) 1606497..1606988 - 492 WP_001138904.1 nucleotide binding protein YajQ -
QEN27_RS07985 (1607156) 1607156..1608067 + 912 WP_000705847.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T278366 WP_000227784.1 NZ_CP123008:1603064-1603606 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT278366 WP_001297137.1 NZ_CP123008:1602769-1603080 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References