Detailed information of TA system TA027835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4855571..4856613
• Replicon: chromosome
• Accession: NC_017911
• Organism: Pseudomonas fluorescens A506

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: PFLA506_RS21745
• Protein ID: WP_014719841.1
• Coordinates: 4855571..4856146 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A0W0MVK8
• Locus tag: PFLA506_RS21750
• Protein ID: WP_014719842.1
• Coordinates: 4856143..4856613 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PFLA506_RS27310	4850596..4851414	+	819	WP_044483421.1	hypothetical protein	-
PFLA506_RS21715	4851630..4851959	+	330	WP_014719835.1	hypothetical protein	-
PFLA506_RS21720	4851930..4852568	-	639	WP_014719836.1	LysE family transporter	-
PFLA506_RS21725	4852595..4853401	-	807	WP_014719837.1	2OG-Fe(II) oxygenase family protein	-
PFLA506_RS21730	4853626..4854318	+	693	WP_014719838.1	helix-turn-helix domain-containing protein	-
PFLA506_RS21735	4854327..4854962	-	636	WP_014719839.1	LysE family translocator	-
PFLA506_RS21740	4855119..4855490	+	372	WP_014719840.1	PH domain-containing protein	-
PFLA506_RS21745	4855571..4856146	-	576	WP_014719841.1	PIN domain-containing protein	Toxin
PFLA506_RS21750	4856143..4856613	-	471	WP_014719842.1	helix-turn-helix domain-containing protein	Antitoxin
PFLA506_RS21755	4856808..4857962	+	1155	WP_014719843.1	S-type pyocin domain-containing protein	-
PFLA506_RS21760	4857970..4858227	+	258	WP_014719844.1	colicin transporter	-
PFLA506_RS21765	4858286..4858570	+	285	WP_014719845.1	barstar family protein	-
PFLA506_RS21770	4858591..4858965	-	375	WP_014719846.1	cytochrome c family protein	-
PFLA506_RS21775	4858996..4859427	-	432	WP_014719847.1	VOC family protein	-
PFLA506_RS21780	4859592..4859954	+	363	WP_003194033.1	response regulator	-
PFLA506_RS21785	4860167..4860781	+	615	WP_014719848.1	biliverdin-producing heme oxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4845366..4858227	12861

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21409.76 Da
• Isoelectric Point: 4.7036

>T27835 WP_014719841.1 NC_017911:c4856146-4855571 [Pseudomonas fluorescens A506]
MRCSPFTAVYDACVLYPAALRDLLMWLGLSGLYRARWTGQIQDEWKRNVLKNRPDLTAAQVDCTSALMDIAIPDALVTGY
EDLCSSLDLPDPDDRHVLAAAIRSKAEVFVTFNLKDFPPHILAAYDIEPMHPDDFISDLWDLDQAAVLGAAQKQRRALKK
PAMEAGSYLEMLMRQGLVQTSKAMGPYEMML

Nucleotide

• Length: 576 bp

>T27835 NC_017911:c4856146-4855571 [Pseudomonas fluorescens A506]
ATGAGGTGTTCGCCCTTTACCGCGGTGTACGATGCTTGTGTTCTCTATCCTGCGGCACTTAGGGACTTACTCATGTGGCT
TGGTTTGTCTGGTTTGTATCGAGCGCGATGGACTGGCCAGATTCAGGATGAGTGGAAGCGCAACGTACTTAAGAACCGAC
CCGATCTTACGGCGGCTCAGGTGGACTGCACGTCGGCGCTAATGGACATTGCTATACCTGACGCGTTGGTGACGGGATAT
GAGGATCTGTGTTCGAGTTTGGATTTACCAGACCCGGATGACCGGCATGTGTTGGCGGCGGCAATTCGGAGTAAAGCTGA
GGTTTTCGTGACCTTCAATTTGAAAGACTTTCCACCTCATATATTGGCTGCCTATGACATTGAACCGATGCATCCGGATG
ACTTTATTTCGGATCTCTGGGATTTGGATCAGGCGGCAGTGTTGGGAGCGGCGCAGAAGCAGAGGAGGGCGTTGAAGAAG
CCCGCGATGGAGGCGGGAAGTTATCTTGAGATGTTGATGCGGCAGGGCTTGGTGCAGACTAGCAAGGCAATGGGACCCTA
TGAAATGATGCTGTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17496.17 Da
• Isoelectric Point: 6.1100

>AT27835 WP_014719842.1 NC_017911:c4856613-4856143 [Pseudomonas fluorescens A506]
MTINNLPKTILPLEKEVEAAVQGQRELASLLSTKFETQRIDIFDKEDKPHRLVLPTSALRLLVDILGELALGNAVKVVPV
HAELTSQEAADLLNVSRPHLVKMLEEGAIPFTKTGRHRRVRFSDLMAFKQRRDEQSQEAMEALVQQAQELGMGYDG

Nucleotide

• Length: 471 bp

>AT27835 NC_017911:c4856613-4856143 [Pseudomonas fluorescens A506]
ATGACCATCAACAATCTTCCCAAAACGATCCTTCCGCTGGAAAAAGAAGTCGAGGCGGCAGTGCAAGGTCAGCGTGAATT
AGCGTCCCTATTGTCGACCAAATTTGAAACCCAGCGCATCGATATCTTCGACAAAGAAGACAAGCCACACAGGCTTGTCC
TACCAACCTCCGCACTGCGTCTGCTGGTCGATATTCTGGGCGAATTGGCGCTGGGCAATGCCGTCAAGGTAGTGCCGGTT
CATGCCGAGTTGACCTCGCAGGAGGCTGCCGACCTGCTTAATGTGTCACGCCCGCATTTGGTCAAGATGCTGGAGGAAGG
CGCCATCCCCTTTACAAAAACGGGCCGACATCGGCGAGTCCGTTTTTCGGATTTGATGGCGTTCAAGCAACGGCGTGACG
AGCAAAGCCAAGAGGCAATGGAAGCGCTGGTTCAACAGGCCCAAGAGTTGGGAATGGGGTATGACGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A0W0MVK8