Detailed information of TA system TA027830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1604402..1604988
• Replicon: chromosome
• Accession: NC_017911
• Organism: Pseudomonas fluorescens A506

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: PFLA506_RS07110
• Protein ID: WP_014717392.1
• Coordinates: 1604680..1604988 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: PFLA506_RS07105
• Protein ID: WP_014717391.1
• Coordinates: 1604402..1604683 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PFLA506_RS28310	1600594..1601130	+	537	WP_144269766.1	hypothetical protein	-
PFLA506_RS07080	1601319..1601621	+	303	WP_044483042.1	hypothetical protein	-
PFLA506_RS07085	1601858..1602352	+	495	WP_202962332.1	hypothetical protein	-
PFLA506_RS07090	1602352..1602594	+	243	WP_014717388.1	hypothetical protein	-
PFLA506_RS07095	1602612..1603304	+	693	WP_014717389.1	SOS response-associated peptidase	-
PFLA506_RS07100	1603377..1603940	+	564	WP_044483044.1	hypothetical protein	-
PFLA506_RS28315	1604027..1604434	+	408	WP_144269767.1	hypothetical protein	-
PFLA506_RS07105	1604402..1604683	-	282	WP_014717391.1	putative addiction module antidote protein	Antitoxin
PFLA506_RS07110	1604680..1604988	-	309	WP_014717392.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PFLA506_RS07115	1605046..1605441	-	396	WP_014717393.1	hypothetical protein	-
PFLA506_RS07120	1605438..1605800	-	363	WP_044483046.1	hypothetical protein	-
PFLA506_RS07125	1605800..1606321	-	522	WP_014717394.1	hypothetical protein	-
PFLA506_RS07130	1606332..1606766	-	435	WP_014717395.1	hypothetical protein	-
PFLA506_RS07135	1606834..1607904	-	1071	WP_014717396.1	hypothetical protein	-
PFLA506_RS07140	1607906..1608526	-	621	WP_014717397.1	HEPN domain-containing protein	-
PFLA506_RS07145	1608598..1609287	-	690	WP_044483048.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1581962..1655824	73862

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-89)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11532.13 Da
• Isoelectric Point: 9.0774

>T27830 WP_014717392.1 NC_017911:c1604988-1604680 [Pseudomonas fluorescens A506]
MNTLITTTEFNQWLKGLKDDRARARIVSRMVSAQEGNFGDCEPVGDGISEMRIFVGPGYRVYYTRRGEVVYLLLCGGDKS
SQKRDIRAAKAILNEIDRGERT

Nucleotide

• Length: 309 bp

>T27830 NC_017911:c1604988-1604680 [Pseudomonas fluorescens A506]
ATGAATACACTCATCACAACAACAGAATTCAACCAGTGGTTGAAAGGCTTGAAGGACGACAGGGCACGAGCCCGGATCGT
CTCGCGGATGGTTTCAGCACAGGAAGGTAATTTCGGTGACTGTGAGCCAGTAGGTGATGGGATTTCAGAGATGCGGATCT
TCGTCGGCCCCGGCTACCGCGTCTACTACACGCGCCGTGGAGAGGTTGTGTATCTGTTACTGTGCGGTGGTGACAAGTCG
TCGCAAAAGCGCGACATAAGGGCCGCAAAAGCTATCCTAAACGAGATCGATCGTGGAGAACGTACATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10265.75 Da
• Isoelectric Point: 5.8233

>AT27830 WP_014717391.1 NC_017911:c1604683-1604402 [Pseudomonas fluorescens A506]
MTQLKPFEASEYLTNEDTISEFLTASLEDPNPDVFLRALQEVAKARGMSQLARDAGMNRESLYKALAPGAKPRYDTVLKL
VNALGVKLTAQHV

Nucleotide

• Length: 282 bp

>AT27830 NC_017911:c1604683-1604402 [Pseudomonas fluorescens A506]
ATGACCCAGCTCAAGCCATTTGAGGCGTCTGAATACCTTACTAACGAGGACACCATCAGTGAATTCCTCACTGCCTCGCT
TGAAGACCCAAACCCTGACGTGTTCCTGCGCGCCTTGCAAGAAGTCGCCAAGGCCCGTGGTATGTCGCAGTTGGCCCGTG
ATGCAGGCATGAACCGTGAGAGCCTGTACAAAGCGTTGGCGCCAGGTGCAAAGCCCCGGTATGACACAGTGTTGAAGCTT
GTGAATGCATTAGGCGTGAAGCTGACGGCACAGCACGTTTAG