Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4597909..4598406 Replicon chromosome
Accession NZ_CP122938
Organism Escherichia coli strain ETEC1701

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QDX07_RS22745 Protein ID WP_001173343.1
Coordinates 4597909..4598082 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QDX07_RS22750 Protein ID WP_000398619.1
Coordinates 4598110..4598406 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDX07_RS22735 (4594579) 4594579..4596036 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
QDX07_RS22740 (4596273) 4596273..4597790 + 1518 WP_001295074.1 lysine--tRNA ligase -
QDX07_RS22745 (4597909) 4597909..4598082 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QDX07_RS22750 (4598110) 4598110..4598406 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QDX07_RS22755 (4598633) 4598633..4598905 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
QDX07_RS22760 (4598917) 4598917..4599147 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
QDX07_RS22765 (4599327) 4599327..4600958 + 1632 WP_001216482.1 sensor histidine kinase -
QDX07_RS22770 (4600955) 4600955..4601674 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4559155..4598406 39251


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T278280 WP_001173343.1 NZ_CP122938:c4598082-4597909 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T278280 NZ_CP122938:c4598082-4597909 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT278280 WP_000398619.1 NZ_CP122938:c4598406-4598110 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT278280 NZ_CP122938:c4598406-4598110 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGATTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References