Detailed information of TA system TA027825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2144083..2144718
• Replicon: chromosome
• Accession: NC_017910
• Organism: Shimwellia blattae DSM 4481 = NBRC 105725

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EBL_RS19895
• Protein ID: WP_071840850.1
• Coordinates: 2144083..2144265 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: I2B9F6
• Locus tag: EBL_RS09995
• Protein ID: WP_002440805.1
• Coordinates: 2144305..2144718 (+)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBL_RS09960	2140697..2141158	-	462	WP_002440814.1	phage terminase small subunit P27 family	-
EBL_RS09965	2141273..2141626	-	354	WP_002440812.1	HNH endonuclease	-
EBL_RS09970	2141623..2142051	-	429	WP_002440811.1	hypothetical protein	-
EBL_RS09975	2142055..2142327	-	273	WP_002440809.1	hypothetical protein	-
EBL_RS09980	2142444..2142914	-	471	WP_014716030.1	lysis protein	-
EBL_RS09985	2142923..2143555	-	633	WP_002440807.1	glycoside hydrolase family 19 protein	-
EBL_RS09990	2143559..2143897	-	339	WP_002440806.1	phage holin, lambda family	-
EBL_RS19895	2144083..2144265	+	183	WP_071840850.1	type II toxin-antitoxin system HicA family toxin	Toxin
EBL_RS09995	2144305..2144718	+	414	WP_002440805.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EBL_RS10000	2144805..2145251	+	447	WP_002440804.1	hypothetical protein	-
EBL_RS10005	2145751..2145936	-	186	WP_014716031.1	site-specific DNA-methyltransferase	-
EBL_RS10015	2146062..2147149	+	1088	WP_126298247.1	IS3 family transposase	-
EBL_RS10020	2147152..2148006	-	855	WP_014716032.1	site-specific DNA-methyltransferase	-
EBL_RS10025	2148418..2148987	-	570	WP_002443626.1	DUF1133 family protein	-
EBL_RS20575	2148971..2149129	-	159	WP_014716034.1	YlcG family protein	-
EBL_RS19905	2149086..2149484	-	399	WP_002443625.1	RusA family crossover junction endodeoxyribonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2113724..2179859	66135

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6868.11 Da
• Isoelectric Point: 11.0007

>T27825 WP_071840850.1 NC_017910:2144083-2144265 [Shimwellia blattae DSM 4481 = NBRC 105725]
MKSSELISLLEKNGWVLERIKGSHHQFSHPDFAIVITVPHPRKDVKPGTLRQILKNAKLK

Nucleotide

• Length: 183 bp

>T27825 NC_017910:2144083-2144265 [Shimwellia blattae DSM 4481 = NBRC 105725]
CTGAAGAGCTCGGAACTGATATCACTACTGGAGAAAAATGGCTGGGTCCTGGAGCGCATCAAAGGCAGCCATCACCAGTT
CTCCCATCCCGATTTTGCTATTGTCATTACTGTTCCGCATCCCAGAAAAGACGTGAAGCCAGGAACGCTCCGTCAGATCC
TGAAGAATGCGAAGCTAAAATGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15229.08 Da
• Isoelectric Point: 4.8087

>AT27825 WP_002440805.1 NC_017910:2144305-2144718 [Shimwellia blattae DSM 4481 = NBRC 105725]
MLYPAFVEVDKDGTASGWFPDVPGCLFAGDNMEAAFADARSAIDAHFELLSEKDLPIPGAHPMEEHVVNDPGTYTGGRWL
YVDVNMDKFDGRAERINITLPHRLLERIDSTVKHKPEYGSRSAFLAVAARNELHKSD

Nucleotide

• Length: 414 bp

>AT27825 NC_017910:2144305-2144718 [Shimwellia blattae DSM 4481 = NBRC 105725]
ATGTTATATCCCGCATTCGTTGAGGTTGATAAAGACGGTACCGCCAGTGGCTGGTTTCCTGACGTCCCGGGATGCCTGTT
CGCCGGTGACAATATGGAAGCCGCGTTTGCCGATGCCCGCAGCGCCATAGATGCACACTTTGAGTTGCTGAGTGAAAAGG
ATCTGCCCATTCCCGGGGCTCACCCTATGGAAGAGCATGTTGTGAATGATCCGGGCACTTATACCGGTGGCCGCTGGTTA
TACGTTGACGTCAACATGGATAAGTTCGATGGCCGCGCTGAACGCATCAACATAACCCTTCCCCATCGTCTTCTTGAACG
CATTGATTCAACGGTTAAGCACAAACCAGAGTACGGCAGCCGCAGCGCCTTTCTTGCCGTTGCCGCAAGGAATGAACTCC
ACAAATCGGATTGA

Structures

Antitoxin

• AlphaFold DB: I2B9F6