278107

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 97161..97964 Replicon plasmid unnamed8
Accession NZ_CP122867
Organism Escherichia coli strain ETEC1720

Toxin (Protein)


Gene name KacT Uniprot ID A0A0K3X5E3
Locus tag QDY31_RS26025 Protein ID WP_000348884.1
Coordinates 97434..97964 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID G3CAI7
Locus tag QDY31_RS26020 Protein ID WP_001275013.1
Coordinates 97161..97430 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDY31_RS25995 (92566) 92566..92826 - 261 Protein_101 transposase -
QDY31_RS26000 (92842) 92842..94112 + 1271 WP_169029802.1 IS3-like element IS2 family transposase -
QDY31_RS26005 (94087) 94087..94281 + 195 WP_077881938.1 hypothetical protein -
QDY31_RS26010 (94402) 94402..95136 + 735 WP_282836067.1 tyrosine-type recombinase/integrase -
QDY31_RS26015 (95284) 95284..96273 - 990 WP_024201093.1 RepB family plasmid replication initiator protein -
QDY31_RS26020 (97161) 97161..97430 + 270 WP_001275013.1 DUF1778 domain-containing protein Antitoxin
QDY31_RS26025 (97434) 97434..97964 + 531 WP_000348884.1 GNAT family N-acetyltransferase Toxin
QDY31_RS26030 (97965) 97965..98182 + 218 Protein_108 transposase -
QDY31_RS26035 (98241) 98241..98891 - 651 Protein_109 transposase zinc-binding domain-containing protein -
QDY31_RS26040 (98872) 98872..99144 - 273 WP_000019163.1 hypothetical protein -
QDY31_RS26045 (99215) 99215..99302 - 88 Protein_111 hypothetical protein -
QDY31_RS26050 (99330) 99330..99545 + 216 WP_001435544.1 RepB family plasmid replication initiator protein -
QDY31_RS26055 (100393) 100393..101025 + 633 WP_000312330.1 ParA family protein -
QDY31_RS26060 (101025) 101025..101399 + 375 WP_063078193.1 hypothetical protein -
QDY31_RS26065 (101637) 101637..102611 + 975 WP_021534625.1 plasmid segregation protein ParM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - estIa / hlyD / hlyD / hlyB / hlyB / hlyA / hlyC 1..107570 107570
- inside IScluster/Tn - - 89111..98891 9780


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20320.22 Da        Isoelectric Point: 6.7486

>T278107 WP_000348884.1 NZ_CP122867:97434-97964 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEVLFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10229.63 Da        Isoelectric Point: 6.4702

>AT278107 WP_001275013.1 NZ_CP122867:97161-97430 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K3X5E3

Antitoxin

Source ID Structure
AlphaFold DB G3CAI7

References