Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4232328..4232825 Replicon chromosome
Accession NZ_CP122859
Organism Escherichia coli strain ETEC1720

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QDY31_RS20850 Protein ID WP_001173343.1
Coordinates 4232328..4232501 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QDY31_RS20855 Protein ID WP_000398619.1
Coordinates 4232529..4232825 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDY31_RS20830 (4227737) 4227737..4229173 + 1437 WP_013097853.1 undecaprenyl-phosphate galactose phosphotransferase WbaP -
QDY31_RS20835 (4229324) 4229324..4230730 + 1407 WP_196010848.1 NADP-dependent phosphogluconate dehydrogenase -
QDY31_RS20840 (4230823) 4230823..4231134 + 312 Protein_4076 GDP-mannose 4,6-dehydratase -
QDY31_RS20845 (4231295) 4231295..4232263 + 969 WP_172951730.1 IS5-like element IS903B family transposase -
QDY31_RS20850 (4232328) 4232328..4232501 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QDY31_RS20855 (4232529) 4232529..4232825 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QDY31_RS20860 (4233052) 4233052..4233324 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
QDY31_RS20865 (4233336) 4233336..4233566 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
QDY31_RS20870 (4233747) 4233747..4235378 + 1632 WP_001216477.1 sensor histidine kinase -
QDY31_RS20875 (4235375) 4235375..4236094 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gnd 4218432..4232825 14393
- flank IS/Tn - - 4231340..4232263 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T278096 WP_001173343.1 NZ_CP122859:c4232501-4232328 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT278096 WP_000398619.1 NZ_CP122859:c4232825-4232529 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References