Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 97162..97965 Replicon plasmid unnamed7
Accession NZ_CP122851
Organism Escherichia coli strain ETEC1722

Toxin (Protein)


Gene name KacT Uniprot ID A0A0K3X5E3
Locus tag QDX00_RS25455 Protein ID WP_000348884.1
Coordinates 97435..97965 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID G3CAI7
Locus tag QDX00_RS25450 Protein ID WP_001275013.1
Coordinates 97162..97431 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDX00_RS25425 (92567) 92567..92827 - 261 Protein_101 transposase -
QDX00_RS25430 (92843) 92843..94113 + 1271 WP_169029802.1 IS3-like element IS2 family transposase -
QDX00_RS25435 (94088) 94088..94282 + 195 WP_077881938.1 hypothetical protein -
QDX00_RS25440 (94403) 94403..95137 + 735 WP_282836067.1 tyrosine-type recombinase/integrase -
QDX00_RS25445 (95285) 95285..96274 - 990 WP_024201093.1 RepB family plasmid replication initiator protein -
QDX00_RS25450 (97162) 97162..97431 + 270 WP_001275013.1 DUF1778 domain-containing protein Antitoxin
QDX00_RS25455 (97435) 97435..97965 + 531 WP_000348884.1 GNAT family N-acetyltransferase Toxin
QDX00_RS25460 (97966) 97966..98183 + 218 Protein_108 transposase -
QDX00_RS25465 (98242) 98242..98892 - 651 Protein_109 transposase zinc-binding domain-containing protein -
QDX00_RS25470 (98873) 98873..99145 - 273 WP_000019163.1 hypothetical protein -
QDX00_RS25475 (99216) 99216..99303 - 88 Protein_111 hypothetical protein -
QDX00_RS25480 (99331) 99331..99546 + 216 WP_001435544.1 RepB family plasmid replication initiator protein -
QDX00_RS25485 (100394) 100394..101026 + 633 WP_000312330.1 ParA family protein -
QDX00_RS25490 (101026) 101026..101400 + 375 WP_063078193.1 hypothetical protein -
QDX00_RS25495 (101638) 101638..102612 + 975 WP_021534625.1 plasmid segregation protein ParM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - estIa / hlyD / hlyB / hlyB / hlyA / hlyC 1..107571 107571
- inside IScluster/Tn - - 89112..98892 9780


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20320.22 Da        Isoelectric Point: 6.7486

>T278078 WP_000348884.1 NZ_CP122851:97435-97965 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEVLFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10229.63 Da        Isoelectric Point: 6.4702

>AT278078 WP_001275013.1 NZ_CP122851:97162-97431 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K3X5E3

Antitoxin

Source ID Structure
AlphaFold DB G3CAI7

References