Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4229305..4229802 Replicon chromosome
Accession NZ_CP122844
Organism Escherichia coli strain ETEC1722

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QDX00_RS20795 Protein ID WP_001173343.1
Coordinates 4229305..4229478 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QDX00_RS20800 Protein ID WP_000398619.1
Coordinates 4229506..4229802 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDX00_RS20775 (4224714) 4224714..4226150 + 1437 WP_013097853.1 undecaprenyl-phosphate galactose phosphotransferase WbaP -
QDX00_RS20780 (4226301) 4226301..4227707 + 1407 WP_196010848.1 NADP-dependent phosphogluconate dehydrogenase -
QDX00_RS20785 (4227800) 4227800..4228111 + 312 Protein_4065 GDP-mannose 4,6-dehydratase -
QDX00_RS20790 (4228272) 4228272..4229240 + 969 WP_172951730.1 IS5-like element IS903B family transposase -
QDX00_RS20795 (4229305) 4229305..4229478 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QDX00_RS20800 (4229506) 4229506..4229802 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QDX00_RS20805 (4230029) 4230029..4230301 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
QDX00_RS20810 (4230313) 4230313..4230543 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
QDX00_RS20815 (4230724) 4230724..4232355 + 1632 WP_001216477.1 sensor histidine kinase -
QDX00_RS20820 (4232352) 4232352..4233071 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gnd 4215409..4229802 14393
- flank IS/Tn - - 4228317..4229240 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T278068 WP_001173343.1 NZ_CP122844:c4229478-4229305 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT278068 WP_000398619.1 NZ_CP122844:c4229802-4229506 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References