Detailed information of TA system TA027804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 74327..74886
• Replicon: plasmid pO157
• Accession: NC_017907
• Organism: Escherichia coli Xuzhou21

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: CDCO157_RS27925
• Protein ID: WP_000421248.1
• Coordinates: 74611..74886 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: CDCO157_RS27920
• Protein ID: WP_001178089.1
• Coordinates: 74327..74611 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDCO157_RS27880	69518..70078	+	561	WP_000840472.1	fertility inhibition protein FinO	-
CDCO157_RS27885	70211..70423	+	213	WP_001302189.1	ANR family transcriptional regulator	-
CDCO157_RS27890	70668..71129	+	462	WP_001233853.1	thermonuclease family protein	-
CDCO157_RS27895	71175..71384	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
CDCO157_RS27900	71422..71760	+	339	WP_000766796.1	hypothetical protein	-
CDCO157_RS27905	72000..72254	+	255	WP_000083831.1	replication regulatory protein RepA	-
CDCO157_RS27910	72490..72564	+	75	WP_001370046.1	RepA leader peptide Tap	-
CDCO157_RS27915	72557..73414	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
CDCO157_RS27920	74327..74611	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
CDCO157_RS27925	74611..74886	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDCO157_RS27930	74981..75187	+	207	WP_001105064.1	hypothetical protein	-
CDCO157_RS28515	75287..75502	-	216	Protein_79	transposase	-
CDCO157_RS30780	75546..76112	-	567	Protein_80	IS3-like element IS600 family transposase	-
CDCO157_RS27945	76112..76528	-	417	Protein_81	IS91 family transposase	-
CDCO157_RS27950	76528..76713	-	186	WP_001302179.1	hypothetical protein	-
CDCO157_RS27955	76890..79100	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
CDCO157_RS27960	79144..79533	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP	1..92728	92728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T27804 WP_000421248.1 NC_017907:74611-74886 [Escherichia coli Xuzhou21]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T27804 NC_017907:74611-74886 [Escherichia coli Xuzhou21]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT27804 WP_001178089.1 NC_017907:74327-74611 [Escherichia coli Xuzhou21]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT27804 NC_017907:74327-74611 [Escherichia coli Xuzhou21]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8