Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5095013..5095510 Replicon chromosome
Accession NC_017906
Organism Escherichia coli Xuzhou21

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CDCO157_RS26110 Protein ID WP_001173343.1
Coordinates 5095337..5095510 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CDCO157_RS26105 Protein ID WP_000398619.1
Coordinates 5095013..5095309 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDCO157_RS26080 5091744..5092463 - 720 WP_000611288.1 two-component system response regulator DcuR -
CDCO157_RS26085 5092460..5094091 - 1632 WP_001216477.1 sensor histidine kinase -
CDCO157_RS26090 5094272..5094502 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
CDCO157_RS26095 5094514..5094786 + 273 WP_000405647.1 N-acetyltransferase -
CDCO157_RS26105 5095013..5095309 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CDCO157_RS26110 5095337..5095510 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CDCO157_RS26115 5095629..5097146 - 1518 WP_001295074.1 lysine--tRNA ligase -
CDCO157_RS26120 5097383..5098840 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T27797 WP_001173343.1 NC_017906:5095337-5095510 [Escherichia coli Xuzhou21]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T27797 NC_017906:5095337-5095510 [Escherichia coli Xuzhou21]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT27797 WP_000398619.1 NC_017906:5095013-5095309 [Escherichia coli Xuzhou21]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT27797 NC_017906:5095013-5095309 [Escherichia coli Xuzhou21]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References