Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4225459..4226035 Replicon chromosome
Accession NZ_CP122816
Organism Escherichia coli strain ETEC1731

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QDY29_RS20695 Protein ID WP_001297643.1
Coordinates 4225748..4226035 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QDY29_RS20690 Protein ID WP_000063156.1
Coordinates 4225459..4225761 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDY29_RS20675 (4222055) 4222055..4224205 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QDY29_RS20680 (4224255) 4224255..4224458 + 204 WP_000467859.1 YbdD/YjiX family protein -
QDY29_RS20685 (4224469) 4224469..4225425 + 957 WP_001297640.1 GTPase -
QDY29_RS20690 (4225459) 4225459..4225761 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QDY29_RS20695 (4225748) 4225748..4226035 - 288 WP_001297643.1 BrnT family toxin Toxin
QDY29_RS20700 (4226234) 4226234..4226931 - 698 Protein_4047 IS1 family transposase -
QDY29_RS20705 (4226988) 4226988..4227083 + 96 Protein_4048 dienelactone hydrolase family protein -
QDY29_RS20710 (4227229) 4227229..4228461 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
QDY29_RS20715 (4228502) 4228502..4229782 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T277944 WP_001297643.1 NZ_CP122816:c4226035-4225748 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT277944 WP_000063156.1 NZ_CP122816:c4225761-4225459 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References