Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 208993..210616 Replicon plasmid unnamed1
Accession NZ_CP122769
Organism Escherichia coli strain ETEC1738

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag QDY55_RS28820 Protein ID WP_000088046.1
Coordinates 209291..210616 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag QDY55_RS28815 Protein ID WP_024136327.1
Coordinates 208993..209286 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDY55_RS28785 (QDY55_28780) 204865..206106 + 1242 WP_001053338.1 VWA domain-containing protein -
QDY55_RS28790 (QDY55_28785) 206197..206328 + 132 WP_000374059.1 hypothetical protein -
QDY55_RS28795 (QDY55_28790) 206669..206950 - 282 WP_000077926.1 hypothetical protein -
QDY55_RS28800 (QDY55_28795) 207000..207191 - 192 WP_000398480.1 hypothetical protein -
QDY55_RS28805 (QDY55_28800) 207283..207624 - 342 WP_001151575.1 hypothetical protein -
QDY55_RS28810 (QDY55_28805) 207997..208389 + 393 WP_000341066.1 hypothetical protein -
QDY55_RS28815 (QDY55_28810) 208993..209286 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
QDY55_RS28820 (QDY55_28815) 209291..210616 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
QDY55_RS28825 (QDY55_28820) 210677..210883 + 207 WP_000134171.1 hypothetical protein -
QDY55_RS28830 (QDY55_28825) 210985..211395 + 411 WP_282870909.1 hypothetical protein -
QDY55_RS28835 (QDY55_28830) 211408..212223 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
QDY55_RS28840 (QDY55_28835) 212477..212626 + 150 WP_156839068.1 hypothetical protein -
QDY55_RS28845 (QDY55_28840) 212655..213352 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
QDY55_RS28850 (QDY55_28845) 213511..214176 - 666 WP_000490638.1 hypothetical protein -
QDY55_RS28855 (QDY55_28850) 214234..214614 - 381 WP_001426317.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / mcr-1.1 - 1..232987 232987
- flank IS/Tn - - 212655..212930 275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T277883 WP_000088046.1 NZ_CP122769:209291-210616 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT277883 WP_024136327.1 NZ_CP122769:208993-209286 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References