Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 97073..97876 Replicon plasmid unnamed3
Accession NZ_CP122761
Organism Escherichia coli strain ETEC1739

Toxin (Protein)


Gene name KacT Uniprot ID A0A0K3X5E3
Locus tag QDW95_RS24035 Protein ID WP_000348884.1
Coordinates 97346..97876 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID G3CAI7
Locus tag QDW95_RS24030 Protein ID WP_001275013.1
Coordinates 97073..97342 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDW95_RS24005 (92478) 92478..92738 - 261 Protein_101 transposase -
QDW95_RS24010 (92754) 92754..94024 + 1271 WP_169029802.1 IS3-like element IS2 family transposase -
QDW95_RS24015 (93999) 93999..94193 + 195 WP_077881938.1 hypothetical protein -
QDW95_RS24020 (94314) 94314..95048 + 735 WP_282836067.1 tyrosine-type recombinase/integrase -
QDW95_RS24025 (95196) 95196..96185 - 990 WP_024201093.1 RepB family plasmid replication initiator protein -
QDW95_RS24030 (97073) 97073..97342 + 270 WP_001275013.1 DUF1778 domain-containing protein Antitoxin
QDW95_RS24035 (97346) 97346..97876 + 531 WP_000348884.1 GNAT family N-acetyltransferase Toxin
QDW95_RS24040 (97877) 97877..98094 + 218 Protein_108 transposase -
QDW95_RS24045 (98153) 98153..98803 - 651 Protein_109 transposase zinc-binding domain-containing protein -
QDW95_RS24050 (98784) 98784..99056 - 273 WP_000019163.1 hypothetical protein -
QDW95_RS24055 (99127) 99127..99214 - 88 Protein_111 hypothetical protein -
QDW95_RS24060 (99242) 99242..99457 + 216 WP_001435544.1 RepB family plasmid replication initiator protein -
QDW95_RS24065 (100305) 100305..100937 + 633 WP_000312330.1 ParA family protein -
QDW95_RS24070 (100937) 100937..101311 + 375 WP_063078193.1 hypothetical protein -
QDW95_RS24075 (101549) 101549..102523 + 975 WP_021534625.1 plasmid segregation protein ParM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyD / hlyB / hlyA / hlyC 1..106236 106236
- inside IScluster/Tn - - 89023..98803 9780


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20320.22 Da        Isoelectric Point: 6.7486

>T277850 WP_000348884.1 NZ_CP122761:97346-97876 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEVLFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10229.63 Da        Isoelectric Point: 6.4702

>AT277850 WP_001275013.1 NZ_CP122761:97073-97342 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K3X5E3

Antitoxin

Source ID Structure
AlphaFold DB G3CAI7

References