Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4294547..4295044 Replicon chromosome
Accession NZ_CP122758
Organism Escherichia coli strain ETEC1739

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QDW95_RS21230 Protein ID WP_001173343.1
Coordinates 4294547..4294720 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QDW95_RS21235 Protein ID WP_000398619.1
Coordinates 4294748..4295044 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDW95_RS21210 (4289956) 4289956..4291392 + 1437 WP_013097853.1 undecaprenyl-phosphate galactose phosphotransferase WbaP -
QDW95_RS21215 (4291543) 4291543..4292949 + 1407 WP_196010848.1 NADP-dependent phosphogluconate dehydrogenase -
QDW95_RS21220 (4293042) 4293042..4293353 + 312 Protein_4152 GDP-mannose 4,6-dehydratase -
QDW95_RS21225 (4293514) 4293514..4294482 + 969 WP_172951730.1 IS5-like element IS903B family transposase -
QDW95_RS21230 (4294547) 4294547..4294720 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QDW95_RS21235 (4294748) 4294748..4295044 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QDW95_RS21240 (4295271) 4295271..4295543 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
QDW95_RS21245 (4295555) 4295555..4295785 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
QDW95_RS21250 (4295966) 4295966..4297597 + 1632 WP_001216477.1 sensor histidine kinase -
QDW95_RS21255 (4297594) 4297594..4298313 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gnd 4280395..4295044 14649
- flank IS/Tn - - 4293559..4294482 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T277847 WP_001173343.1 NZ_CP122758:c4294720-4294547 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT277847 WP_000398619.1 NZ_CP122758:c4295044-4294748 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References