Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4363197..4363773 Replicon chromosome
Accession NZ_CP122685
Organism Escherichia coli strain ETEC4067

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QDX30_RS21485 Protein ID WP_001297643.1
Coordinates 4363486..4363773 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag QDX30_RS21480 Protein ID WP_000063160.1
Coordinates 4363197..4363499 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDX30_RS21465 (4359793) 4359793..4361943 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QDX30_RS21470 (4361993) 4361993..4362196 + 204 WP_000467859.1 YbdD/YjiX family protein -
QDX30_RS21475 (4362207) 4362207..4363163 + 957 WP_001297640.1 GTPase -
QDX30_RS21480 (4363197) 4363197..4363499 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
QDX30_RS21485 (4363486) 4363486..4363773 - 288 WP_001297643.1 BrnT family toxin Toxin
QDX30_RS21490 (4363972) 4363972..4364669 - 698 Protein_4206 IS1 family transposase -
QDX30_RS21495 (4364726) 4364726..4364821 + 96 Protein_4207 dienelactone hydrolase family protein -
QDX30_RS21500 (4364967) 4364967..4366199 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
QDX30_RS21505 (4366240) 4366240..4367520 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - cheD 4317272..4364475 47203


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T277709 WP_001297643.1 NZ_CP122685:c4363773-4363486 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT277709 WP_000063160.1 NZ_CP122685:c4363499-4363197 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References