Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4494315..4494812 Replicon chromosome
Accession NZ_CP122681
Organism Escherichia coli strain ETEC4068

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QDW67_RS22005 Protein ID WP_001173343.1
Coordinates 4494315..4494488 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QDW67_RS22010 Protein ID WP_000398619.1
Coordinates 4494516..4494812 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDW67_RS21995 (4490985) 4490985..4492442 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
QDW67_RS22000 (4492679) 4492679..4494196 + 1518 WP_001295074.1 lysine--tRNA ligase -
QDW67_RS22005 (4494315) 4494315..4494488 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QDW67_RS22010 (4494516) 4494516..4494812 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QDW67_RS22015 (4495039) 4495039..4495311 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
QDW67_RS22020 (4495323) 4495323..4495553 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
QDW67_RS22025 (4495733) 4495733..4497364 + 1632 WP_001216482.1 sensor histidine kinase -
QDW67_RS22030 (4497361) 4497361..4498080 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4459575..4494812 35237


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T277691 WP_001173343.1 NZ_CP122681:c4494488-4494315 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT277691 WP_000398619.1 NZ_CP122681:c4494812-4494516 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References