Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4269277..4269853 Replicon chromosome
Accession NZ_CP122681
Organism Escherichia coli strain ETEC4068

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QDW67_RS20840 Protein ID WP_001297643.1
Coordinates 4269566..4269853 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag QDW67_RS20835 Protein ID WP_000063160.1
Coordinates 4269277..4269579 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDW67_RS20820 (4265873) 4265873..4268023 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QDW67_RS20825 (4268073) 4268073..4268276 + 204 WP_000467859.1 YbdD/YjiX family protein -
QDW67_RS20830 (4268287) 4268287..4269243 + 957 WP_001297640.1 GTPase -
QDW67_RS20835 (4269277) 4269277..4269579 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
QDW67_RS20840 (4269566) 4269566..4269853 - 288 WP_001297643.1 BrnT family toxin Toxin
QDW67_RS20845 (4270052) 4270052..4270749 - 698 Protein_4077 IS1 family transposase -
QDW67_RS20850 (4270806) 4270806..4270901 + 96 Protein_4078 dienelactone hydrolase family protein -
QDW67_RS20855 (4271047) 4271047..4272279 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
QDW67_RS20860 (4272320) 4272320..4273600 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T277690 WP_001297643.1 NZ_CP122681:c4269853-4269566 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT277690 WP_000063160.1 NZ_CP122681:c4269579-4269277 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References