Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4546377..4546874 Replicon chromosome
Accession NZ_CP122679
Organism Escherichia coli strain ETEC4069

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QDW53_RS22495 Protein ID WP_001173343.1
Coordinates 4546377..4546550 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QDW53_RS22500 Protein ID WP_000398619.1
Coordinates 4546578..4546874 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDW53_RS22485 (4543047) 4543047..4544504 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
QDW53_RS22490 (4544741) 4544741..4546258 + 1518 WP_001295074.1 lysine--tRNA ligase -
QDW53_RS22495 (4546377) 4546377..4546550 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QDW53_RS22500 (4546578) 4546578..4546874 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QDW53_RS22505 (4547101) 4547101..4547373 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
QDW53_RS22510 (4547385) 4547385..4547615 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
QDW53_RS22515 (4547795) 4547795..4549426 + 1632 WP_001216482.1 sensor histidine kinase -
QDW53_RS22520 (4549423) 4549423..4550142 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4507580..4546874 39294


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T277671 WP_001173343.1 NZ_CP122679:c4546550-4546377 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT277671 WP_000398619.1 NZ_CP122679:c4546874-4546578 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References