Detailed information of TA system TA027762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 54802..55352
• Replicon: chromosome
• Accession: NC_017906
• Organism: Escherichia coli Xuzhou21

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: CDCO157_RS00270
• Protein ID: WP_001160975.1
• Coordinates: 55038..55352 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: CDCO157_RS00265
• Protein ID: WP_000125566.1
• Coordinates: 54802..55035 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDCO157_RS00240	49876..50163	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
CDCO157_RS00245	50221..51552	+	1332	WP_001183177.1	MFS transporter	-
CDCO157_RS00250	51660..52190	+	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
CDCO157_RS00255	52183..54045	+	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
CDCO157_RS00260	54237..54716	+	480	WP_000624372.1	type 3 dihydrofolate reductase	-
CDCO157_RS00265	54802..55035	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
CDCO157_RS00270	55038..55352	+	315	WP_001160975.1	CcdB family protein	Toxin
CDCO157_RS00275	55349..56197	-	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
CDCO157_RS00280	56204..56581	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
CDCO157_RS00285	56584..57405	-	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
CDCO157_RS00290	57402..58391	-	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
CDCO157_RS00295	58391..59677	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T27762 WP_001160975.1 NC_017906:55038-55352 [Escherichia coli Xuzhou21]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T27762 NC_017906:55038-55352 [Escherichia coli Xuzhou21]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT27762 WP_000125566.1 NC_017906:54802-55035 [Escherichia coli Xuzhou21]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT27762 NC_017906:54802-55035 [Escherichia coli Xuzhou21]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9