277588

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 2828762..2830482 Replicon chromosome
Accession NZ_CP122660
Organism Escherichia coli strain ETEC4073

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag QDY26_RS13995 Protein ID WP_000312833.1
Coordinates 2828762..2829418 (+) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag QDY26_RS14000 Protein ID WP_000634203.1
Coordinates 2829415..2830482 (+) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDY26_RS13980 (2824747) 2824747..2824923 + 177 WP_001056893.1 hypothetical protein -
QDY26_RS13985 (2824963) 2824963..2827400 + 2438 Protein_2734 type I restriction-modification system subunit M -
QDY26_RS13990 (2827400) 2827400..2828749 + 1350 WP_000228011.1 restriction endonuclease subunit S -
QDY26_RS13995 (2828762) 2828762..2829418 + 657 WP_000312833.1 toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT Toxin
QDY26_RS14000 (2829415) 2829415..2830482 + 1068 WP_000634203.1 toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG Antitoxin
QDY26_RS14005 (2830501) 2830501..2833596 + 3096 WP_001688437.1 HsdR family type I site-specific deoxyribonuclease -
QDY26_RS14010 (2833596) 2833596..2834312 + 717 WP_001122107.1 SprT family zinc-dependent metalloprotease -
QDY26_RS14015 (2834534) 2834534..2834659 - 126 WP_021501707.1 hypothetical protein -
QDY26_RS14020 (2834783) 2834783..2835157 - 375 WP_000171781.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - csgB / csgD / csgE / csgF / csgG 2805220..2835157 29937


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T277588 WP_000312833.1 NZ_CP122660:2828762-2829418 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT277588 WP_000634203.1 NZ_CP122660:2829415-2830482 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References