Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4315715..4316291 Replicon chromosome
Accession NZ_CP122652
Organism Escherichia coli strain ETEC4075

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QDW61_RS21200 Protein ID WP_001297643.1
Coordinates 4316004..4316291 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag QDW61_RS21195 Protein ID WP_000063160.1
Coordinates 4315715..4316017 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDW61_RS21180 (4312311) 4312311..4314461 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QDW61_RS21185 (4314511) 4314511..4314714 + 204 WP_000467859.1 YbdD/YjiX family protein -
QDW61_RS21190 (4314725) 4314725..4315681 + 957 WP_001297640.1 GTPase -
QDW61_RS21195 (4315715) 4315715..4316017 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
QDW61_RS21200 (4316004) 4316004..4316291 - 288 WP_001297643.1 BrnT family toxin Toxin
QDW61_RS21205 (4316490) 4316490..4317187 - 698 Protein_4149 IS1 family transposase -
QDW61_RS21210 (4317244) 4317244..4317339 + 96 Protein_4150 dienelactone hydrolase family protein -
QDW61_RS21215 (4317485) 4317485..4318717 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
QDW61_RS21220 (4318758) 4318758..4320038 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T277542 WP_001297643.1 NZ_CP122652:c4316291-4316004 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT277542 WP_000063160.1 NZ_CP122652:c4316017-4315715 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References