Detailed information of TA system TA027752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 2558045..2558580
• Replicon: chromosome
• Accession: NC_017857
• Organism: Methylophaga nitratireducenticrescens

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A7U5TVG9
• Locus tag: Q7A_RS12115
• Protein ID: WP_041354673.1
• Coordinates: 2558284..2558580 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A7U5TVF5
• Locus tag: Q7A_RS12110
• Protein ID: WP_041354672.1
• Coordinates: 2558045..2558284 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q7A_RS12075	2553292..2553801	-	510	WP_041354668.1	hypothetical protein	-
Q7A_RS12080	2553888..2554463	-	576	WP_014707894.1	antibiotic biosynthesis monooxygenase	-
Q7A_RS12085	2554559..2554858	-	300	WP_052501231.1	hypothetical protein	-
Q7A_RS12090	2554848..2555045	-	198	WP_014707895.1	hypothetical protein	-
Q7A_RS12095	2555265..2556737	-	1473	WP_202971532.1	hypothetical protein	-
Q7A_RS12100	2556892..2557596	-	705	WP_014707897.1	NYN domain-containing protein	-
Q7A_RS12110	2558045..2558284	+	240	WP_041354672.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
Q7A_RS12115	2558284..2558580	+	297	WP_041354673.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Q7A_RS12120	2558616..2558909	-	294	WP_041354675.1	HigA family addiction module antidote protein	-
Q7A_RS12125	2558909..2559187	-	279	WP_041354677.1	type II toxin-antitoxin system RelE/ParE family toxin	-
Q7A_RS12130	2559292..2559786	-	495	WP_041354678.1	ATP-binding protein	-
Q7A_RS12135	2559866..2560051	-	186	WP_014707902.1	BrnA antitoxin family protein	-
Q7A_RS12140	2560068..2560382	-	315	WP_014707903.1	HigA family addiction module antidote protein	-
Q7A_RS12145	2560393..2560671	-	279	WP_041354680.1	type II toxin-antitoxin system RelE/ParE family toxin	-
Q7A_RS12150	2560882..2561214	+	333	WP_014707905.1	hypothetical protein	-
Q7A_RS12155	2561295..2562077	+	783	WP_014707906.1	multicopper oxidase domain-containing protein	-
Q7A_RS12160	2562064..2562792	+	729	WP_014707907.1	copper resistance protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Antitoxin ParD (1-76)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11532.29 Da
• Isoelectric Point: 8.4904

>T27752 WP_041354673.1 NC_017857:2558284-2558580 [Methylophaga nitratireducenticrescens]
MPSFKLSRKAKADLKSIALYTEREWGRDQRNHYILQFDHCFKLLVENPKLGQSCDEISLGYRQHLQGSHVIFYQQNAEGT
IEIIRILHKNMLPEGYLI

Nucleotide

• Length: 297 bp

>T27752 NC_017857:2558284-2558580 [Methylophaga nitratireducenticrescens]
ATGCCAAGTTTCAAGCTTTCCAGGAAGGCCAAAGCTGATCTCAAATCTATAGCCCTTTACACGGAACGTGAATGGGGTCG
GGATCAACGAAACCACTACATTCTTCAGTTTGATCATTGCTTCAAGCTTTTGGTTGAGAATCCGAAGTTGGGGCAAAGCT
GCGATGAAATAAGTCTGGGTTATCGTCAACACCTTCAGGGCAGCCATGTTATTTTCTACCAACAGAACGCTGAAGGTACT
ATTGAGATTATCCGTATCCTCCACAAGAACATGTTGCCTGAAGGATATCTGATTTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9070.97 Da
• Isoelectric Point: 4.8615

>AT27752 WP_041354672.1 NC_017857:2558045-2558284 [Methylophaga nitratireducenticrescens]
MHKNTSVSLGQHFDTFISEQLKSGRYSSTSEVVRAGLRLLEESETRLTTLRKLLKEGEDSGFEEYSYDAFIRELDNEGH

Nucleotide

• Length: 240 bp

>AT27752 NC_017857:2558045-2558284 [Methylophaga nitratireducenticrescens]
ATGCATAAGAACACTAGCGTTAGTCTTGGCCAACATTTTGATACATTCATTTCTGAACAACTCAAAAGTGGCCGATACAG
CTCAACAAGTGAGGTTGTTCGTGCAGGATTGCGATTGCTTGAAGAGTCTGAAACTAGACTCACCACACTTCGAAAATTGC
TTAAAGAAGGCGAAGATAGTGGTTTCGAGGAATATTCCTATGATGCTTTTATTCGTGAACTGGATAATGAAGGACACTAA

Structures

Toxin

• AlphaFold DB: A0A7U5TVG9

Antitoxin

• AlphaFold DB: A0A7U5TVF5