Detailed information of TA system TA027751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relB/YoeB-YefM
• Location: 938200..938702
• Replicon: chromosome
• Accession: NC_017857
• Organism: Methylophaga nitratireducenticrescens

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: I1XH97
• Locus tag: Q7A_RS04490
• Protein ID: WP_014706141.1
• Coordinates: 938200..938454 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: I1XH98
• Locus tag: Q7A_RS04495
• Protein ID: WP_014706142.1
• Coordinates: 938451..938702 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q7A_RS15165	933315..933467	+	153	WP_014706133.1	hypothetical protein	-
Q7A_RS04445	933590..934501	+	912	WP_014706134.1	hypothetical protein	-
Q7A_RS04450	934642..934875	+	234	WP_014706135.1	hypothetical protein	-
Q7A_RS04455	934862..935128	+	267	WP_041354373.1	type II toxin-antitoxin system RelE/ParE family toxin	-
Q7A_RS04460	935165..935380	+	216	WP_041354375.1	TIGR02450 family Trp-rich protein	-
Q7A_RS04465	935445..935783	+	339	WP_014706136.1	thioredoxin family protein	-
Q7A_RS04470	936006..936230	+	225	WP_202971552.1	type II toxin-antitoxin system RelE/ParE family toxin	-
Q7A_RS04475	936220..936510	+	291	WP_202971553.1	helix-turn-helix domain-containing protein	-
Q7A_RS04480	936552..937397	-	846	WP_014706139.1	DNA ligase	-
Q7A_RS04485	937800..938138	+	339	WP_014706140.1	thioredoxin family protein	-
Q7A_RS04490	938200..938454	-	255	WP_014706141.1	Txe/YoeB family addiction module toxin	Toxin
Q7A_RS04495	938451..938702	-	252	WP_014706142.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
Q7A_RS04500	939110..940957	+	1848	WP_014706143.1	lytic transglycosylase domain-containing protein	-
Q7A_RS04505	941199..942329	-	1131	WP_014706144.1	PepSY domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.78 Da
• Isoelectric Point: 9.9936

>T27751 WP_014706141.1 NC_017857:c938454-938200 [Methylophaga nitratireducenticrescens]
MKLIFSENAWEDYLYWQKTDKKILNRINKLIKETKRQPFDGIGKPEALKHGLAGYWSRRINDEHRMVYKVTDDALLLAQL
RYHY

Nucleotide

• Length: 255 bp

>T27751 NC_017857:c938454-938200 [Methylophaga nitratireducenticrescens]
ATGAAACTCATCTTCTCAGAAAACGCCTGGGAAGATTATCTGTACTGGCAGAAAACTGACAAAAAAATCTTGAACCGAAT
CAACAAGCTGATCAAGGAAACGAAAAGACAACCTTTCGACGGCATCGGCAAACCAGAAGCACTCAAACACGGCCTCGCTG
GTTACTGGTCCCGCCGAATTAACGACGAGCACCGCATGGTCTACAAGGTCACGGATGATGCATTGCTCCTTGCCCAGCTC
CGATACCACTACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9315.52 Da
• Isoelectric Point: 4.4488

>AT27751 WP_014706142.1 NC_017857:c938702-938451 [Methylophaga nitratireducenticrescens]
MDAISYTAARTNLAKTMEQVCEDHSPVIITRSKSQSVVMISLDDYEALQETTYLLRVPKNARRLLESVTELEQGGGQEKD
LVE

Nucleotide

• Length: 252 bp

>AT27751 NC_017857:c938702-938451 [Methylophaga nitratireducenticrescens]
ATGGATGCCATCAGCTACACAGCCGCTAGAACCAACCTAGCAAAAACTATGGAACAGGTCTGTGAAGACCATTCACCAGT
TATTATCACCCGGAGCAAGTCGCAATCCGTGGTAATGATCTCTCTTGACGATTATGAGGCACTGCAAGAAACTACATATC
TGTTGCGCGTACCAAAGAACGCTCGTCGTTTGCTGGAATCCGTTACCGAGCTGGAGCAAGGCGGCGGCCAGGAAAAGGAT
CTTGTTGAATGA

Structures

Toxin

• AlphaFold DB: I1XH97

Antitoxin

• AlphaFold DB: I1XH98