Detailed information of TA system TA027748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 807401..807911
• Replicon: chromosome
• Accession: NC_017857
• Organism: Methylophaga nitratireducenticrescens

Toxin (Protein)

• Gene name: relB
• Uniprot ID: I1XGX3
• Locus tag: Q7A_RS03820
• Protein ID: WP_014706017.1
• Coordinates: 807401..807664 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: I1XGX4
• Locus tag: Q7A_RS03825
• Protein ID: WP_014706018.1
• Coordinates: 807657..807911 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q7A_RS03795	802886..803680	-	795	WP_014706012.1	UDP-2,3-diacylglucosamine diphosphatase	-
Q7A_RS03800	803820..805859	+	2040	WP_041354334.1	oligopeptidase A	-
Q7A_RS03805	805948..806364	-	417	WP_014706014.1	hypothetical protein	-
Q7A_RS03810	806624..806890	-	267	WP_014706016.1	DksA/TraR family C4-type zinc finger protein	-
Q7A_RS03820	807401..807664	-	264	WP_014706017.1	Txe/YoeB family addiction module toxin	Toxin
Q7A_RS03825	807657..807911	-	255	WP_014706018.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
Q7A_RS03830	808548..808772	-	225	WP_041354338.1	hypothetical protein	-
Q7A_RS03835	809034..810518	+	1485	WP_014706020.1	4-hydroxy-3-polyprenylbenzoate decarboxylase	-
Q7A_RS03840	810507..810878	-	372	WP_041354340.1	MAPEG family protein	-
Q7A_RS03845	810939..812687	-	1749	WP_014706022.1	OFA family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10408.85 Da
• Isoelectric Point: 9.4084

>T27748 WP_014706017.1 NC_017857:c807664-807401 [Methylophaga nitratireducenticrescens]
MTKRLLSWTDEAWKDYLYWQGQDKKTLRRINKLITDVQRSPFEGIGKPEALKENLSGFWSRRIDDTHRLVYAVEDSAITI
VSCRYHY

Nucleotide

• Length: 264 bp

>T27748 NC_017857:c807664-807401 [Methylophaga nitratireducenticrescens]
ATGACTAAACGCTTATTGTCATGGACTGACGAGGCTTGGAAAGATTATCTCTACTGGCAAGGTCAGGATAAAAAAACACT
ACGCCGAATCAACAAATTGATCACCGATGTTCAACGCAGTCCCTTTGAAGGGATCGGCAAACCGGAGGCCTTAAAAGAAA
ATCTATCCGGTTTCTGGTCGCGACGGATTGATGATACCCATCGCTTGGTTTATGCCGTTGAGGATTCAGCGATTACGATT
GTTTCTTGTCGGTATCATTATTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9464.81 Da
• Isoelectric Point: 5.6920

>AT27748 WP_014706018.1 NC_017857:c807911-807657 [Methylophaga nitratireducenticrescens]
MKIVSFSEARNSLKTVLDGVVNDADTTIITRRDAEDAVVMSLDYFNGLMETVHLLRSPENVKHLNKSIEQFKAGKAKPRE
LMDD

Nucleotide

• Length: 255 bp

>AT27748 NC_017857:c807911-807657 [Methylophaga nitratireducenticrescens]
ATGAAAATCGTCTCATTTTCTGAAGCCAGAAATAGCTTAAAAACCGTTCTTGATGGTGTGGTGAACGATGCAGATACCAC
CATCATCACCCGCCGTGATGCAGAAGATGCCGTGGTGATGTCGTTGGATTACTTCAACGGCCTGATGGAAACCGTGCATT
TACTGCGCTCACCGGAGAACGTAAAACATCTGAATAAATCGATTGAACAATTCAAAGCTGGCAAGGCGAAACCGCGAGAG
CTGATGGATGACTAA

Structures

Toxin

• AlphaFold DB: I1XGX3

Antitoxin

• AlphaFold DB: I1XGX4