Detailed information of TA system TA027747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 700458..702024
• Replicon: chromosome
• Accession: NC_017857
• Organism: Methylophaga nitratireducenticrescens

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: I1XGM4
• Locus tag: Q7A_RS03305
• Protein ID: WP_014705918.1
• Coordinates: 700458..701771 (-)
• Length: 438 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: Q7A_RS03310
• Protein ID: WP_014705919.1
• Coordinates: 701773..702024 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q7A_RS03280	696143..696994	+	852	WP_014705913.1	OmpA family protein	-
Q7A_RS03285	697396..697587	+	192	WP_014705914.1	CsbD family protein	-
Q7A_RS03290	697803..698363	+	561	WP_202971549.1	BON domain-containing protein	-
Q7A_RS03295	698371..698832	+	462	WP_014705916.1	hypothetical protein	-
Q7A_RS03300	699053..700279	+	1227	WP_014705917.1	sigma-54 dependent transcriptional regulator	-
Q7A_RS03305	700458..701771	-	1314	WP_014705918.1	type II toxin-antitoxin system HipA family toxin	Toxin
Q7A_RS03310	701773..702024	-	252	WP_014705919.1	helix-turn-helix transcriptional regulator	Antitoxin
Q7A_RS15115	702269..702427	+	159	Protein_652	IS982 family transposase	-
Q7A_RS03315	702429..702818	+	390	WP_041354298.1	hypothetical protein	-
Q7A_RS03325	703366..703719	+	354	WP_014705922.1	hypothetical protein	-
Q7A_RS03330	704099..705169	+	1071	Protein_655	IS3 family transposase	-
Q7A_RS03335	705333..706010	+	678	WP_014705925.1	VTT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	704975..705169	194

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (154-397)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-66)

Sequences

Toxin

Protein

• Length: 438 a.a.
• Molecular weight: 49383.59 Da
• Isoelectric Point: 6.7683

>T27747 WP_014705918.1 NC_017857:c701771-700458 [Methylophaga nitratireducenticrescens]
MGRRQHYIPLDVWISNRIVGQLIRESTGAISFIYDQRWLDWEHAFPVSLSLPLRKDRFIGARVISVFENLLPDNDGVRKR
LAERVGAYGTDAFSLLSVIGRDCIGALQFLPQGSFQETSNQLEGTVLSEHEIEVLLGELDEVPLGLQKENDFRISIAGAQ
GKTALLFHNGEWIKPTGTTPTTHILKPQIGKLSNGMDLTHSVENEFLCLKLLKNFGLRTANTEIIDFGSRRALVIERFDR
RWTADGRLIRLPQEDCCQALSIPPTRKYQSEGGPGIVEIMDLFRGSDEPTQDRMDFFMSTILFWAIGATDGHGKNFSLSL
MPGGRYRMTPLYDVLTLQPSVDAKQVAHKNYKLSMSFGKSRHYKIKEIAVRYIIETGVEAGLSRQSIAEIFEQLCKDKES
VIENTLKELPEEFPKKLLESTFTAFDKNISLLLSNSR

Nucleotide

• Length: 1314 bp

>T27747 NC_017857:c701771-700458 [Methylophaga nitratireducenticrescens]
ATGGGCCGTCGTCAACACTATATCCCGCTTGATGTATGGATAAGTAACCGAATCGTCGGACAGCTTATTCGTGAAAGCAC
AGGCGCTATATCGTTTATATATGATCAACGTTGGTTAGATTGGGAGCATGCTTTTCCTGTTTCTTTATCCTTACCTCTCA
GGAAAGATCGCTTCATCGGTGCTCGGGTAATATCCGTCTTCGAGAACCTTTTACCCGATAATGACGGTGTTAGAAAAAGG
CTTGCAGAACGAGTAGGTGCATATGGCACTGATGCTTTCAGTCTGTTGTCAGTGATTGGCAGAGATTGTATTGGAGCATT
ACAGTTCCTTCCCCAGGGGAGTTTTCAAGAAACAAGTAACCAGCTTGAGGGTACTGTTTTATCAGAGCATGAAATTGAAG
TCTTACTGGGCGAGTTAGATGAGGTACCACTCGGGCTACAAAAAGAAAATGACTTTCGAATATCCATTGCTGGAGCCCAA
GGGAAGACCGCTTTGCTCTTTCATAATGGAGAGTGGATTAAACCCACAGGCACAACTCCAACAACACATATACTCAAGCC
ACAAATAGGCAAACTGTCGAATGGTATGGATCTCACCCACAGTGTTGAGAATGAATTTCTGTGTTTAAAACTCCTAAAAA
ACTTTGGTCTAAGAACAGCTAACACAGAGATCATTGATTTTGGAAGTCGTAGGGCCTTGGTGATTGAACGATTTGATAGG
AGGTGGACTGCGGATGGCAGGCTGATCCGATTGCCACAGGAAGATTGTTGCCAAGCACTCTCCATCCCACCCACGAGAAA
ATATCAAAGTGAAGGAGGTCCAGGTATTGTAGAAATAATGGACTTATTTCGTGGCAGTGATGAGCCAACGCAGGACCGGA
TGGACTTTTTCATGTCCACAATTTTATTTTGGGCTATTGGTGCCACAGATGGCCATGGTAAAAACTTTAGCTTGTCTTTA
ATGCCTGGCGGAAGATATAGAATGACGCCGCTATACGATGTCCTAACCCTACAGCCATCAGTCGATGCCAAACAAGTAGC
TCATAAGAATTACAAGCTCAGTATGAGTTTTGGTAAATCCAGACACTACAAAATTAAAGAAATTGCTGTTCGGTATATCA
TCGAGACTGGCGTTGAAGCAGGGCTTTCACGTCAATCAATCGCTGAAATTTTCGAGCAACTTTGTAAAGACAAAGAATCC
GTCATCGAAAATACGCTAAAAGAGCTTCCTGAAGAATTTCCCAAAAAATTACTGGAATCCACTTTTACAGCATTTGATAA
AAATATTTCACTATTACTAAGTAACTCTCGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9217.60 Da
• Isoelectric Point: 8.7278

>AT27747 WP_014705919.1 NC_017857:c702024-701773 [Methylophaga nitratireducenticrescens]
MKILVRSPKDLGNAIRHARKAKALTQSELASKSGIWQETISKIENGVSSTKLDTVFDLFAALDLEIHVQERSKGNISQLE
EIL

Nucleotide

• Length: 252 bp

>AT27747 NC_017857:c702024-701773 [Methylophaga nitratireducenticrescens]
ATGAAGATACTGGTCAGATCGCCTAAAGATTTAGGTAATGCCATCAGGCACGCTCGTAAAGCGAAAGCCTTAACTCAAAG
TGAGTTAGCCTCAAAAAGCGGTATATGGCAGGAAACGATTTCAAAAATTGAAAATGGTGTCTCGAGCACGAAGCTGGATA
CTGTTTTTGATTTATTTGCCGCTTTGGATTTGGAAATTCATGTACAAGAGCGCAGTAAGGGTAATATCAGCCAGCTAGAG
GAAATCTTGTAG

Structures

Toxin

• AlphaFold DB: I1XGM4