Detailed information of TA system TA027746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-couple_hipB
• Location: 1593372..1593982
• Replicon: chromosome
• Accession: NC_017856
• Organism: Methylophaga frappieri

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: Q7C_RS07605
• Protein ID: WP_187287362.1
• Coordinates: 1593740..1593982 (-)
• Length: 81 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: Q7C_RS07600
• Protein ID: WP_014704153.1
• Coordinates: 1593372..1593743 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q7C_RS07580	1589108..1590280	+	1173	WP_151194744.1	hypothetical protein	-
Q7C_RS07585	1590301..1591191	+	891	WP_014704150.1	HD domain-containing protein	-
Q7C_RS07590	1591433..1592902	+	1470	WP_151194745.1	hypothetical protein	-
Q7C_RS07600	1593372..1593743	-	372	WP_014704153.1	helix-turn-helix domain-containing protein	Antitoxin
Q7C_RS07605	1593740..1593982	-	243	WP_187287362.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Q7C_RS07615	1594455..1595405	+	951	WP_041366665.1	sel1 repeat family protein	-
Q7C_RS07620	1595438..1597738	+	2301	WP_014704157.1	EAL domain-containing protein	-
Q7C_RS07625	1597828..1598256	+	429	WP_014704158.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1463232..1601963	138731

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (1-73)

Antitoxin

• Cro/C1-type helix-turn-helix domain (66-122)

Sequences

Toxin

Protein

• Length: 81 a.a.
• Molecular weight: 8944.40 Da
• Isoelectric Point: 10.8294

>T27746 WP_187287362.1 NC_017856:c1593982-1593740 [Methylophaga frappieri]
VQYSRKAIKGLRKLPANVADQFRTAFQEIANSRGHWEVKKLAGREGYRLRIGGYRGIYKIEGEQVTVLVLAVGPPGGIYK

Nucleotide

• Length: 243 bp

>T27746 NC_017856:c1593982-1593740 [Methylophaga frappieri]
GTGCAGTACAGCAGAAAAGCAATTAAAGGACTTCGGAAACTACCCGCAAACGTTGCTGACCAGTTCCGTACTGCATTTCA
GGAAATAGCTAATAGCCGAGGCCATTGGGAAGTCAAAAAACTGGCAGGACGCGAAGGCTACCGGCTGCGGATTGGCGGGT
ACCGTGGTATTTACAAAATTGAAGGCGAACAGGTAACGGTTCTTGTGTTGGCTGTTGGCCCACCTGGAGGTATCTACAAA
TGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13225.86 Da
• Isoelectric Point: 3.8971

>AT27746 WP_014704153.1 NC_017856:c1593743-1593372 [Methylophaga frappieri]
MSAQIIENNGVPEYAVVPIADYQALIEKAEMLDDVAAFDKAEATLAAGEDELVPSNIVDSLLANVNAVKVWREYRGMSQA
TLADAAGLSQAYIAQIETGKREGRVDAYRAIANVLDVNIDDLV

Nucleotide

• Length: 372 bp

>AT27746 NC_017856:c1593743-1593372 [Methylophaga frappieri]
ATGAGCGCACAGATCATTGAGAACAATGGCGTGCCCGAGTATGCGGTGGTGCCAATTGCCGACTACCAGGCATTAATCGA
AAAGGCTGAAATGCTGGACGATGTAGCCGCTTTTGATAAGGCCGAAGCCACACTGGCGGCAGGTGAGGATGAACTGGTGC
CGTCTAATATCGTTGATTCGTTGCTGGCCAATGTAAACGCGGTGAAAGTTTGGCGTGAATACCGTGGTATGTCACAGGCT
ACTCTGGCTGATGCTGCAGGGCTTTCACAGGCGTACATCGCGCAAATTGAGACCGGAAAGCGTGAAGGACGGGTTGATGC
CTACAGGGCCATTGCCAATGTGCTGGATGTCAATATAGATGACTTGGTGTAA