277458

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4546884..4547460 Replicon chromosome
Accession NZ_CP122634
Organism Escherichia coli strain ETEC4085

Toxin (Protein)


Gene name shpA Uniprot ID A0A2X2XYK1
Locus tag QDW62_RS22795 Protein ID WP_001360091.1
Coordinates 4547173..4547460 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QDW62_RS22790 Protein ID WP_000063156.1
Coordinates 4546884..4547186 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDW62_RS22775 (4543480) 4543480..4545630 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QDW62_RS22780 (4545680) 4545680..4545883 + 204 WP_000467859.1 YbdD/YjiX family protein -
QDW62_RS22785 (4545894) 4545894..4546850 + 957 WP_001297640.1 GTPase -
QDW62_RS22790 (4546884) 4546884..4547186 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QDW62_RS22795 (4547173) 4547173..4547460 - 288 WP_001360091.1 BrnT family toxin Toxin
QDW62_RS22800 (4547659) 4547659..4548356 - 698 Protein_4459 IS1-like element IS1F family transposase -
QDW62_RS22805 (4548413) 4548413..4548508 + 96 Protein_4460 dienelactone hydrolase family protein -
QDW62_RS22810 (4548654) 4548654..4549886 + 1233 WP_015675357.1 multidrug efflux MFS transporter MdtM -
QDW62_RS22815 (4549927) 4549927..4551207 + 1281 WP_001037377.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11325.80 Da        Isoelectric Point: 6.8821

>T277458 WP_001360091.1 NZ_CP122634:c4547460-4547173 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKTDRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT277458 WP_000063156.1 NZ_CP122634:c4547186-4546884 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X2XYK1

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References