Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2546328..2546854 Replicon chromosome
Accession NZ_CP122634
Organism Escherichia coli strain ETEC4085

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag QDW62_RS12745 Protein ID WP_000323025.1
Coordinates 2546567..2546854 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag QDW62_RS12740 Protein ID WP_000534858.1
Coordinates 2546328..2546567 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDW62_RS12715 (2541526) 2541526..2542713 + 1188 WP_000937614.1 IS91-like element IS91 family transposase -
QDW62_RS12720 (2543734) 2543734..2544756 + 1023 Protein_2485 ISNCY family transposase -
QDW62_RS12725 (2544877) 2544877..2545026 + 150 WP_011443592.1 protein YdfW -
QDW62_RS12730 (2545463) 2545463..2545795 - 333 WP_001301033.1 FlxA-like family protein -
QDW62_RS12735 (2545998) 2545998..2546303 - 306 WP_001326990.1 protein YdfV -
QDW62_RS12740 (2546328) 2546328..2546567 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
QDW62_RS12745 (2546567) 2546567..2546854 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
QDW62_RS12750 (2546926) 2546926..2547081 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
QDW62_RS12755 (2547298) 2547298..2547549 + 252 WP_000980994.1 protein Rem -
QDW62_RS12760 (2547616) 2547616..2547894 + 279 WP_012304870.1 hypothetical protein -
QDW62_RS12765 (2547896) 2547896..2548945 + 1050 WP_001424248.1 DUF968 domain-containing protein -
QDW62_RS12770 (2548959) 2548959..2549711 + 753 WP_001047136.1 antitermination protein -
QDW62_RS12775 (2549989) 2549989..2550078 - 90 WP_120795389.1 hypothetical protein -
QDW62_RS12780 (2550133) 2550133..2550345 - 213 WP_000087756.1 cold shock-like protein CspF -
QDW62_RS12785 (2550646) 2550646..2550861 + 216 WP_000066484.1 cold shock-like protein CspB -
QDW62_RS12790 (2551225) 2551225..2551395 - 171 WP_001405948.1 putative zinc-binding protein YnfU -
QDW62_RS12795 (2551615) 2551615..2551830 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2519636..2589244 69608
- flank IS/Tn - - 2541526..2542713 1187
- inside Prophage - - 2527355..2589244 61889


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T277451 WP_000323025.1 NZ_CP122634:2546567-2546854 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT277451 WP_000534858.1 NZ_CP122634:2546328-2546567 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References