Detailed information of TA system TA027743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 572289..574011
• Replicon: chromosome
• Accession: NC_017856
• Organism: Methylophaga frappieri

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: I1YFL1
• Locus tag: Q7C_RS02565
• Protein ID: WP_014703134.1
• Coordinates: 572289..573227 (-)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: Q7C_RS02570
• Protein ID: WP_041366844.1
• Coordinates: 573220..574011 (-)
• Length: 264 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q7C_RS02545	568230..568742	-	513	WP_014703130.1	S26 family signal peptidase	-
Q7C_RS02550	568726..569073	-	348	WP_014703131.1	hypothetical protein	-
Q7C_RS02555	569066..571465	-	2400	WP_014703132.1	type IV secretion system protein TraC	-
Q7C_RS02560	571465..572157	-	693	WP_014703133.1	DsbC family protein	-
Q7C_RS02565	572289..573227	-	939	WP_014703134.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
Q7C_RS02570	573220..574011	-	792	WP_041366844.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
Q7C_RS02575	574231..574617	-	387	WP_000180225.1	hypothetical protein	-
Q7C_RS02580	574614..575186	-	573	WP_001944091.1	type IV conjugative transfer system lipoprotein TraV	-
Q7C_RS02585	575261..576550	-	1290	WP_014703136.1	TraB/VirB10 family protein	-
Q7C_RS02590	576550..577449	-	900	WP_014703137.1	type-F conjugative transfer system secretin TraK	-
Q7C_RS02595	577433..578059	-	627	WP_000667170.1	hypothetical protein	-
Q7C_RS02600	578056..578337	-	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
Q7C_RS02605	578518..578841	+	324	WP_001896384.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	376210..618754	242544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-279)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-97)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 35608.87 Da
• Isoelectric Point: 4.9081

>T27743 WP_014703134.1 NC_017856:c573227-572289 [Methylophaga frappieri]
MDKDSPYYKQVSLLIRMLPVVATETVFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPRDEALINVRAALQRITDRINTQ
PDIRAAFQDNKADELRIVVSSPVATIKIEVSPVARGTLHSAEIMPVQESVEDEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLGSEGISREILVGFLTYTLSHPRPINEVMSPNWQPLNEKFQTEFDGMTFEKVECEDLASVRPMMLIELQK
HFTERDHAFLMSFKRGQPDWALFDYPNAADLPAIRWKLQNINKLAKNQTKHQEQLDKLKQVLDDWLVNANAE

Nucleotide

• Length: 939 bp

>T27743 NC_017856:c573227-572289 [Methylophaga frappieri]
ATGGATAAAGATAGCCCATATTACAAACAGGTTTCCTTGCTCATAAGAATGCTTCCTGTGGTAGCAACAGAGACGGTTTT
TGCACTTAAAGGTGGCACCGCCATTAATTTATTTGTGAGAGATTTTCCTCGGTTATCTGTAGATATTGATCTTGCTTATC
TTCCACTTGAACCAAGAGATGAGGCTTTGATTAATGTCAGGGCTGCATTGCAGCGCATTACAGACAGAATCAATACTCAA
CCAGATATCAGAGCGGCATTTCAGGATAATAAAGCTGATGAACTGAGAATAGTTGTATCAAGTCCGGTTGCGACGATCAA
AATTGAAGTGTCGCCCGTCGCCAGAGGTACATTGCATAGTGCAGAAATAATGCCAGTTCAAGAGTCTGTTGAAGACGAGT
TTGGTTATGCTGAGATTCAGGTAGTCAGTCTTCCCGATCTGTATGGTGGGAAATTGTGTGCTGCAATGGATCGCCAACAT
CCTCGCGACTTATTTGATGTGCGTATGTTACTTGGCAGTGAAGGGATTTCGAGAGAGATTTTGGTGGGTTTTTTAACCTA
TACATTAAGTCACCCTCGGCCTATTAATGAAGTCATGTCGCCAAACTGGCAGCCTCTGAATGAGAAATTTCAGACAGAAT
TTGACGGAATGACTTTTGAAAAAGTTGAATGCGAAGACTTAGCCTCTGTTAGGCCCATGATGTTAATTGAACTGCAAAAA
CATTTCACAGAAAGGGATCATGCTTTCCTTATGTCATTCAAAAGAGGGCAACCTGACTGGGCTTTGTTTGACTATCCTAA
TGCAGCAGACTTGCCAGCGATTCGTTGGAAGTTGCAGAACATTAACAAATTGGCAAAGAATCAAACAAAACATCAAGAGC
AATTAGATAAATTAAAGCAAGTGCTTGATGATTGGCTTGTTAACGCAAACGCCGAGTAA

Antitoxin

Protein

• Length: 264 a.a.
• Molecular weight: 30113.45 Da
• Isoelectric Point: 8.7205

>AT27743 WP_041366844.1 NC_017856:c574011-573220 [Methylophaga frappieri]
MSSKINWLVAHTSPGALVLQQWLTENGVSYSLAQKYAQNGWLKKLCSGVYYRPDAQGDIKPTWVDAIQALDVQLGVPVHL
AGLSSLTHQGLSHYLQLNKEQVWICVKNKSSLPKWFREFPYQNWFYCGNHKLEVNPEKDLKRITVKEKELTVSCAELAAY
EVVDGIGKLISFEHVAELFQGLVNLSPRKVQDILERSSSVQANRIFLFLGRYYDHQWVNRIDETRIELGAGKRQVVEKGR
FDERYQITVPEILSVKKGEQHNG

Nucleotide

• Length: 792 bp

>AT27743 NC_017856:c574011-573220 [Methylophaga frappieri]
ATGTCATCTAAGATAAACTGGCTTGTTGCTCATACTTCTCCTGGTGCGCTAGTACTTCAGCAATGGCTGACTGAAAACGG
TGTGAGCTATTCCTTGGCTCAAAAGTACGCGCAGAACGGTTGGCTAAAGAAGCTATGTTCTGGCGTGTATTATCGTCCAG
ATGCGCAGGGCGATATAAAGCCAACTTGGGTTGATGCCATTCAAGCATTGGATGTGCAATTGGGCGTTCCAGTTCATTTG
GCTGGATTGAGCAGCTTAACTCACCAAGGACTGAGTCACTATTTACAGCTGAACAAAGAGCAAGTTTGGATTTGCGTTAA
AAATAAGTCGTCCTTACCAAAATGGTTTCGTGAATTCCCTTATCAGAATTGGTTTTACTGTGGAAATCATAAGCTTGAAG
TGAATCCCGAGAAAGATTTGAAAAGGATCACGGTTAAAGAGAAAGAACTCACTGTCAGCTGTGCAGAACTTGCTGCCTAT
GAAGTGGTAGACGGGATTGGGAAGCTGATTTCATTTGAGCATGTCGCAGAATTATTTCAGGGTTTAGTCAATCTTAGCCC
TAGAAAAGTACAAGATATTCTTGAGCGAAGCAGCTCTGTTCAGGCAAACCGAATATTTCTATTTCTGGGTCGATACTACG
ATCACCAGTGGGTCAATCGCATAGATGAAACAAGAATTGAGTTGGGTGCAGGAAAGCGGCAGGTTGTCGAAAAAGGACGT
TTTGATGAGCGATATCAAATCACAGTGCCAGAGATATTAAGCGTCAAAAAAGGTGAACAACATAATGGATAA

Structures

Toxin

• AlphaFold DB: I1YFL1