Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1018133..1018716 Replicon chromosome
Accession NZ_CP122629
Organism Escherichia coli strain ETEC4088

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag QDW58_RS04915 Protein ID WP_000254745.1
Coordinates 1018381..1018716 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag QDW58_RS04910 Protein ID WP_000581937.1
Coordinates 1018133..1018381 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDW58_RS04900 (1014472) 1014472..1015773 + 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
QDW58_RS04905 (1015821) 1015821..1018055 + 2235 WP_000226815.1 GTP pyrophosphokinase -
QDW58_RS04910 (1018133) 1018133..1018381 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
QDW58_RS04915 (1018381) 1018381..1018716 + 336 WP_000254745.1 endoribonuclease MazF Toxin
QDW58_RS04920 (1018787) 1018787..1019578 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
QDW58_RS04925 (1019806) 1019806..1021443 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
QDW58_RS04930 (1021531) 1021531..1022829 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T277409 WP_000254745.1 NZ_CP122629:1018381-1018716 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT277409 WP_000581937.1 NZ_CP122629:1018133-1018381 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References