Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4369436..4370012 Replicon chromosome
Accession NZ_CP122604
Organism Escherichia coli strain ETEC6334

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QDW51_RS21540 Protein ID WP_001297643.1
Coordinates 4369725..4370012 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag QDW51_RS21535 Protein ID WP_000063160.1
Coordinates 4369436..4369738 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDW51_RS21520 (4366032) 4366032..4368182 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QDW51_RS21525 (4368232) 4368232..4368435 + 204 WP_000467859.1 YbdD/YjiX family protein -
QDW51_RS21530 (4368446) 4368446..4369402 + 957 WP_001297640.1 GTPase -
QDW51_RS21535 (4369436) 4369436..4369738 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
QDW51_RS21540 (4369725) 4369725..4370012 - 288 WP_001297643.1 BrnT family toxin Toxin
QDW51_RS21545 (4370211) 4370211..4370908 - 698 Protein_4219 IS1 family transposase -
QDW51_RS21550 (4370965) 4370965..4371060 + 96 Protein_4220 dienelactone hydrolase family protein -
QDW51_RS21555 (4371206) 4371206..4372438 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
QDW51_RS21560 (4372479) 4372479..4373759 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T277315 WP_001297643.1 NZ_CP122604:c4370012-4369725 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT277315 WP_000063160.1 NZ_CP122604:c4369738-4369436 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References