Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5302253..5302878 Replicon chromosome
Accession NZ_CP122587
Organism Klebsiella pneumoniae strain NCRE-61

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag QDV36_RS30415 Protein ID WP_002882817.1
Coordinates 5302253..5302636 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag QDV36_RS30420 Protein ID WP_004150355.1
Coordinates 5302636..5302878 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDV36_RS30400 (QDV36_30415) 5299619..5300521 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
QDV36_RS30405 (QDV36_30420) 5300518..5301153 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
QDV36_RS30410 (QDV36_30425) 5301150..5302079 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
QDV36_RS30415 (QDV36_30430) 5302253..5302636 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
QDV36_RS30420 (QDV36_30435) 5302636..5302878 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
QDV36_RS30425 (QDV36_30440) 5303083..5304000 + 918 WP_108714542.1 alpha/beta hydrolase -
QDV36_RS30430 (QDV36_30445) 5304014..5304955 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
QDV36_RS30435 (QDV36_30450) 5305000..5305437 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
QDV36_RS30440 (QDV36_30455) 5305434..5306294 - 861 WP_002882807.1 virulence factor BrkB family protein -
QDV36_RS30445 (QDV36_30460) 5306288..5306887 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T277232 WP_002882817.1 NZ_CP122587:c5302636-5302253 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT277232 WP_004150355.1 NZ_CP122587:c5302878-5302636 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References