Detailed information of TA system TA027723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 545626..546163
• Replicon: chromosome
• Accession: NC_017832
• Organism: Burkholderia pseudomallei 1026b

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BP1026B_RS20790
• Protein ID: WP_009923450.1
• Coordinates: 545626..545895 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BP1026B_RS20795
• Protein ID: WP_004525420.1
• Coordinates: 545879..546163 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BP1026B_RS20765	541541..543244	+	1704	WP_004553173.1	site-specific integrase	-
BP1026B_RS20770	543421..543687	-	267	Protein_443	transposase	-
BP1026B_RS20780	543757..544877	+	1121	WP_038802333.1	IS3 family transposase	-
BP1026B_RS20785	545033..545491	-	459	Protein_445	prohibitin family protein	-
BP1026B_RS20790	545626..545895	+	270	WP_009923450.1	BrnT family toxin	Toxin
BP1026B_RS20795	545879..546163	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BP1026B_RS20800	546810..547193	+	384	Protein_448	DDE-type integrase/transposase/recombinase	-
BP1026B_RS35805	547156..547356	+	201	Protein_449	IS110 family transposase	-
BP1026B_RS35810	548591..548824	+	234	Protein_451	transposase	-
BP1026B_RS20815	549051..549167	-	117	Protein_452	IS3 family transposase	-
BP1026B_RS20820	549433..550893	+	1461	WP_004538070.1	metallophosphoesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	516573..550893	34320
	-	inside	IScluster/Tn	-	-	543421..554536	11115

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10156.52 Da
• Isoelectric Point: 7.1633

>T27723 WP_009923450.1 NC_017832:545626-545895 [Burkholderia pseudomallei 1026b]
MDITFDPTKNKTNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T27723 NC_017832:545626-545895 [Burkholderia pseudomallei 1026b]
ATGGACATCACCTTTGACCCGACCAAGAACAAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT27723 WP_004525420.1 NC_017832:545879-546163 [Burkholderia pseudomallei 1026b]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT27723 NC_017832:545879-546163 [Burkholderia pseudomallei 1026b]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8