Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 47032..47768 Replicon plasmid pKP2185-vir
Accession NZ_CP122567
Organism Klebsiella pneumoniae strain KP2185

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag QDV37_RS00225 Protein ID WP_004098919.1
Coordinates 47286..47768 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag QDV37_RS00220 Protein ID WP_004213599.1
Coordinates 47032..47298 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDV37_RS00195 (QDV37_00195) 42264..42821 + 558 WP_004213590.1 recombinase family protein -
QDV37_RS00200 (QDV37_00200) 42824..45793 + 2970 WP_094936121.1 Tn3 family transposase -
QDV37_RS00205 (QDV37_00205) 45835..46329 + 495 WP_004213594.1 hypothetical protein -
QDV37_RS00210 (QDV37_00210) 46390..46593 + 204 WP_004213596.1 HHA domain-containing protein -
QDV37_RS00215 (QDV37_00215) 46607..46837 + 231 WP_004213598.1 hypothetical protein -
QDV37_RS00220 (QDV37_00220) 47032..47298 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
QDV37_RS00225 (QDV37_00225) 47286..47768 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
QDV37_RS00230 (QDV37_00230) 47969..49372 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
QDV37_RS00235 (QDV37_00235) 49401..50033 - 633 WP_001567369.1 hypothetical protein -
QDV37_RS00240 (QDV37_00240) 50472..51699 + 1228 Protein_47 IS3 family transposase -
QDV37_RS00245 (QDV37_00245) 51695..52090 - 396 Protein_48 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroB / iroC / iroD / iroN / rmpA / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / pla 1..203769 203769
- inside IScluster/Tn - - 42264..51699 9435


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T277176 WP_004098919.1 NZ_CP122567:47286-47768 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT277176 WP_004213599.1 NZ_CP122567:47032-47298 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References