Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 126921..128509 Replicon chromosome
Accession NZ_CP122540
Organism Pseudomonas brenneri strain K5-sn1400

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag QDY63_RS00600 Protein ID WP_223232690.1
Coordinates 127196..128509 (-) Length 438 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5B2V0V9
Locus tag QDY63_RS00595 Protein ID WP_032856701.1
Coordinates 126921..127199 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDY63_RS00565 (QDY63_00565) 122498..123133 + 636 WP_032856710.1 FMN-binding negative transcriptional regulator -
QDY63_RS00570 (QDY63_00570) 123130..123576 + 447 WP_032856709.1 GNAT family N-acetyltransferase -
QDY63_RS00575 (QDY63_00575) 123587..124267 + 681 WP_032856708.1 GNAT family protein -
QDY63_RS00580 (QDY63_00580) 124271..125170 - 900 WP_032856706.1 homocysteine S-methyltransferase family protein -
QDY63_RS00585 (QDY63_00585) 125284..126105 + 822 WP_032856704.1 ABC transporter substrate-binding protein -
QDY63_RS00590 (QDY63_00590) 126102..126917 + 816 WP_065942305.1 amino acid ABC transporter permease -
QDY63_RS00595 (QDY63_00595) 126921..127199 - 279 WP_032856701.1 helix-turn-helix transcriptional regulator Antitoxin
QDY63_RS00600 (QDY63_00600) 127196..128509 - 1314 WP_223232690.1 HipA domain-containing protein Toxin
QDY63_RS00605 (QDY63_00605) 128852..130132 + 1281 WP_032856697.1 autotransporter outer membrane beta-barrel domain-containing protein -
QDY63_RS00610 (QDY63_00610) 130203..132011 - 1809 WP_032856696.1 sodium-extruding oxaloacetate decarboxylase subunit alpha -
QDY63_RS00615 (QDY63_00615) 132023..133438 - 1416 WP_032856695.1 acetyl-CoA carboxylase biotin carboxylase subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - dotU1 / hsiJ1 / fha1 / hsiB1/vipA / hsiC1/vipB / hcp1 / hsiF1 / hsiG1 / hsiH1 / clpV1 / vgrG1a / algB 57485..392140 334655


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(162-385)

Antitoxin

Cro/C1-type helix-turn-helix domain

(27-80)


Sequences

Toxin        


Download         Length: 438 a.a.        Molecular weight: 48330.11 Da        Isoelectric Point: 5.6296

>T277164 WP_223232690.1 NZ_CP122540:c128509-127196 [Pseudomonas brenneri]
MLQIYTEGHWHDAMRLEFSEPQKGFEGPCRFHYRQDYLVENLDDIQQAFSNAVSGRFPLEWDVTFLKTAPAFLHDIAPAG
AAKRFLMRRVGESKPDNVSVDLFLLGRCTPAPIGNLRIKESVEALGARLPIGFARQDVISRDNGFLEYAYEQGAAIGGAT
GAGGEAPKLLMAQSREGLLYPDAILEDADVAQHWFIKFARNGATLNDQAILRSEFHYYKALQALGIETVAAQGLALEEAS
KPSLWMHRFDRNVTATGVERLAVESIYSLAGVITPGSYMDHLEVVQMLADQWIKVGQADEVADLIADYLRRDLLNKILGN
SDNHGRNTSIIRERASVRLAPIYDLAPMVMDAEGITRTTKWPKALEVAGEINWRGVCTALAGFVDPQQAFERLRADAEHL
RALPDLLVASGLPAVTLNHPAIALGKLDQRLKQWGLG

Download         Length: 1314 bp

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10229.94 Da        Isoelectric Point: 11.3766

>AT277164 WP_032856701.1 NZ_CP122540:c127199-126921 [Pseudomonas brenneri]
MTISMDTRALLIESVEQGLAQGTLQIGEAVRRLRVEVTGLHQSQFARMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGL
QMGVVKVRRQTG

Download         Length: 279 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A5B2V0V9

References