Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 32731..33374 Replicon plasmid unnamed2
Accession NZ_CP122509
Organism Escherichia coli strain W444

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag QC806_RS24805 Protein ID WP_001034044.1
Coordinates 32731..33147 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag QC806_RS24810 Protein ID WP_001261286.1
Coordinates 33144..33374 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QC806_RS24785 (28793) 28793..29077 + 285 Protein_37 IS21 family transposase -
QC806_RS24790 (29133) 29133..29830 + 698 WP_223155668.1 IS1-like element IS1A family transposase -
QC806_RS24795 (30084) 30084..31106 - 1023 WP_000361402.1 helicase UvrD -
QC806_RS24800 (31091) 31091..32656 - 1566 WP_001128474.1 AAA family ATPase -
QC806_RS24805 (32731) 32731..33147 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
QC806_RS24810 (33144) 33144..33374 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QC806_RS24815 (33755) 33755..37549 + 3795 WP_001144732.1 hypothetical protein -
QC806_RS24820 (37594) 37594..38010 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
QC806_RS24825 (38007) 38007..38237 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / rmtB / erm(B) / mph(A) / aph(3'')-Ib / aph(6)-Id / tet(A) / floR - 1..121553 121553


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T277119 WP_001034044.1 NZ_CP122509:c33147-32731 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT277119 WP_001261286.1 NZ_CP122509:c33374-33144 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References