Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4281937..4282434 Replicon chromosome
Accession NZ_CP122507
Organism Escherichia coli strain W444

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QC806_RS21055 Protein ID WP_001173343.1
Coordinates 4281937..4282110 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QC806_RS21060 Protein ID WP_000398619.1
Coordinates 4282138..4282434 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QC806_RS21045 (4278607) 4278607..4280064 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
QC806_RS21050 (4280301) 4280301..4281818 + 1518 WP_001295074.1 lysine--tRNA ligase -
QC806_RS21055 (4281937) 4281937..4282110 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QC806_RS21060 (4282138) 4282138..4282434 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QC806_RS21065 (4282661) 4282661..4282933 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
QC806_RS21070 (4282945) 4282945..4283175 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
QC806_RS21075 (4283356) 4283356..4284987 + 1632 WP_001216477.1 sensor histidine kinase -
QC806_RS21080 (4284984) 4284984..4285703 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4246454..4282434 35980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T277115 WP_001173343.1 NZ_CP122507:c4282110-4281937 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT277115 WP_000398619.1 NZ_CP122507:c4282434-4282138 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References