Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4032140..4032716 Replicon chromosome
Accession NZ_CP122507
Organism Escherichia coli strain W444

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QC806_RS19845 Protein ID WP_001297643.1
Coordinates 4032429..4032716 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QC806_RS19840 Protein ID WP_000063156.1
Coordinates 4032140..4032442 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QC806_RS19825 (4028736) 4028736..4030886 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QC806_RS19830 (4030936) 4030936..4031139 + 204 WP_000467859.1 YbdD/YjiX family protein -
QC806_RS19835 (4031150) 4031150..4032106 + 957 WP_001297640.1 GTPase -
QC806_RS19840 (4032140) 4032140..4032442 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QC806_RS19845 (4032429) 4032429..4032716 - 288 WP_001297643.1 BrnT family toxin Toxin
QC806_RS19850 (4032915) 4032915..4033612 - 698 Protein_3879 IS1 family transposase -
QC806_RS19855 (4033669) 4033669..4033764 + 96 Protein_3880 dienelactone hydrolase family protein -
QC806_RS19860 (4033910) 4033910..4035142 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
QC806_RS19865 (4035183) 4035183..4036463 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - cheD 4023379..4033418 10039


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T277112 WP_001297643.1 NZ_CP122507:c4032716-4032429 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT277112 WP_000063156.1 NZ_CP122507:c4032442-4032140 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References