Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4281349..4281846 Replicon chromosome
Accession NZ_CP122499
Organism Escherichia coli strain W409

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QC809_RS21280 Protein ID WP_001173343.1
Coordinates 4281349..4281522 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QC809_RS21285 Protein ID WP_000398619.1
Coordinates 4281550..4281846 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QC809_RS21270 (4278019) 4278019..4279476 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
QC809_RS21275 (4279713) 4279713..4281230 + 1518 WP_063084812.1 lysine--tRNA ligase -
QC809_RS21280 (4281349) 4281349..4281522 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QC809_RS21285 (4281550) 4281550..4281846 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QC809_RS21290 (4282073) 4282073..4282345 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
QC809_RS21295 (4282357) 4282357..4282587 - 231 WP_063084813.1 4Fe-4S mono-cluster protein YjdI -
QC809_RS21300 (4282768) 4282768..4284399 + 1632 WP_001216477.1 sensor histidine kinase -
QC809_RS21305 (4284396) 4284396..4285115 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4285164..4285457 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T277059 WP_001173343.1 NZ_CP122499:c4281522-4281349 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT277059 WP_000398619.1 NZ_CP122499:c4281846-4281550 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References