Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3958358..3958934 Replicon chromosome
Accession NZ_CP122496
Organism Escherichia coli strain a7

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QC805_RS19625 Protein ID WP_001297643.1
Coordinates 3958358..3958645 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QC805_RS19630 Protein ID WP_000063156.1
Coordinates 3958632..3958934 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QC805_RS19605 (3954611) 3954611..3955891 - 1281 WP_001037380.1 DUF445 domain-containing protein -
QC805_RS19610 (3955932) 3955932..3957164 - 1233 WP_284614161.1 multidrug efflux MFS transporter MdtM -
QC805_RS19615 (3957310) 3957310..3957405 - 96 Protein_3834 dienelactone hydrolase family protein -
QC805_RS19620 (3957462) 3957462..3958159 + 698 Protein_3835 IS1 family transposase -
QC805_RS19625 (3958358) 3958358..3958645 + 288 WP_001297643.1 BrnT family toxin Toxin
QC805_RS19630 (3958632) 3958632..3958934 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QC805_RS19635 (3958968) 3958968..3959924 - 957 WP_001297640.1 GTPase -
QC805_RS19640 (3959935) 3959935..3960138 - 204 WP_000467859.1 YbdD/YjiX family protein -
QC805_RS19645 (3960188) 3960188..3962338 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QC805_RS19650 (3962715) 3962715..3962930 + 216 Protein_3841 Tar ligand binding domain-containing protein -
QC805_RS19655 (3962982) 3962982..3963679 + 698 WP_223571425.1 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3963176..3963679 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T277038 WP_001297643.1 NZ_CP122496:3958358-3958645 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT277038 WP_000063156.1 NZ_CP122496:3958632-3958934 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References