Detailed information of TA system TA027701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2170797..2171311
• Replicon: chromosome
• Accession: NC_017763
• Organism: Staphylococcus aureus subsp. aureus HO 5096 0412

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: SAEMRSA15_RS10990
• Protein ID: WP_000581792.1
• Coordinates: 2170797..2171006 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: -
• Locus tag: SAEMRSA15_RS10995
• Protein ID: WP_000138928.1
• Coordinates: 2171018..2171311 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAEMRSA15_RS10970	2166305..2167663	-	1359	WP_000611469.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
SAEMRSA15_RS10975	2167678..2168748	-	1071	WP_000159632.1	D-alanine--D-alanine ligase	-
SAEMRSA15_RS10980	2169066..2170268	+	1203	WP_001109944.1	rod shape-determining protein RodA	-
SAEMRSA15_RS10985	2170532..2170669	-	138	WP_000828354.1	hypothetical protein	-
SAEMRSA15_RS10990	2170797..2171006	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
SAEMRSA15_RS10995	2171018..2171311	-	294	WP_000138928.1	copper-sensing transcriptional repressor CsoR	Antitoxin
SAEMRSA15_RS11000	2171477..2172961	+	1485	WP_000571551.1	cardiolipin synthase	-
SAEMRSA15_RS11005	2172989..2173636	+	648	WP_001187617.1	HD domain-containing protein	-
SAEMRSA15_RS11010	2174114..2174986	-	873	WP_000725802.1	membrane protein insertase YidC	-
SAEMRSA15_RS11015	2175072..2175713	-	642	WP_000483153.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T27701 WP_000581792.1 NC_017763:c2171006-2170797 [Staphylococcus aureus subsp. aureus HO 5096 0412]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T27701 NC_017763:c2171006-2170797 [Staphylococcus aureus subsp. aureus HO 5096 0412]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11184.82 Da
• Isoelectric Point: 6.2243

>AT27701 WP_000138928.1 NC_017763:c2171311-2171018 [Staphylococcus aureus subsp. aureus HO 5096 0412]
MTEQDIAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT27701 NC_017763:c2171311-2171018 [Staphylococcus aureus subsp. aureus HO 5096 0412]
ATGACTGAACAAGATATTGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1