Detailed information of TA system TA027700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2151041..2151570
• Replicon: chromosome
• Accession: NC_017763
• Organism: Staphylococcus aureus subsp. aureus HO 5096 0412

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAEMRSA15_RS10900
• Protein ID: WP_000621175.1
• Coordinates: 2151041..2151403 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: SAEMRSA15_RS10905
• Protein ID: WP_000948330.1
• Coordinates: 2151400..2151570 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAEMRSA15_RS10875	2148019..2148789	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SAEMRSA15_RS10880	2148764..2149243	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SAEMRSA15_RS10885	2149245..2149571	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAEMRSA15_RS10890	2149691..2150692	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAEMRSA15_RS10900	2151041..2151403	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAEMRSA15_RS10905	2151400..2151570	-	171	WP_000948330.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAEMRSA15_RS10910	2151655..2152803	-	1149	WP_001281141.1	alanine racemase	-
SAEMRSA15_RS10915	2152869..2153228	-	360	WP_000581193.1	holo-ACP synthase	-
SAEMRSA15_RS10920	2153232..2153723	-	492	WP_001205918.1	PH domain-containing protein	-
SAEMRSA15_RS10925	2153710..2155293	-	1584	WP_001294624.1	PH domain-containing protein	-
SAEMRSA15_RS10930	2155286..2155765	-	480	WP_001287080.1	hypothetical protein	-
SAEMRSA15_RS10935	2155974..2156534	-	561	WP_001092400.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T27700 WP_000621175.1 NC_017763:c2151403-2151041 [Staphylococcus aureus subsp. aureus HO 5096 0412]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T27700 NC_017763:c2151403-2151041 [Staphylococcus aureus subsp. aureus HO 5096 0412]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATTATTCAAAATGACACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAATATAAATTGGATAAAGACTCAGTCATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTATTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6237.75 Da
• Isoelectric Point: 3.8305

>AT27700 WP_000948330.1 NC_017763:c2151570-2151400 [Staphylococcus aureus subsp. aureus HO 5096 0412]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNESYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT27700 NC_017763:c2151570-2151400 [Staphylococcus aureus subsp. aureus HO 5096 0412]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAATCATATTTATCTTCTAATTCAA
CGAATGAATGA