Detailed information of TA system TA027697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1936499..1937275
• Replicon: chromosome
• Accession: NC_017763
• Organism: Staphylococcus aureus subsp. aureus HO 5096 0412

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: SAEMRSA15_RS09555
• Protein ID: WP_000031112.1
• Coordinates: 1936499..1936651 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: SAEMRSA15_RS09560
• Protein ID: WP_001251224.1
• Coordinates: 1936676..1937275 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAEMRSA15_RS09535	1932405..1933226	+	822	WP_000669382.1	RluA family pseudouridine synthase	-
SAEMRSA15_RS09540	1933688..1935073	-	1386	WP_000116232.1	class II fumarate hydratase	-
SAEMRSA15_RS09545	1935269..1935664	-	396	WP_000901023.1	hypothetical protein	-
SAEMRSA15_RS09555	1936499..1936651	-	153	WP_000031112.1	hypothetical protein	Toxin
SAEMRSA15_RS09560	1936676..1937275	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
SAEMRSA15_RS09565	1937434..1937904	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
SAEMRSA15_RS09570	1937909..1939036	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
SAEMRSA15_RS09575	1939186..1939908	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
SAEMRSA15_RS09580	1939901..1941358	-	1458	WP_000649910.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5966.31 Da
• Isoelectric Point: 3.8962

>T27697 WP_000031112.1 NC_017763:c1936651-1936499 [Staphylococcus aureus subsp. aureus HO 5096 0412]
MSKDKDPKLNYHEEENSMVTDFEDLKELSKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T27697 NC_017763:c1936651-1936499 [Staphylococcus aureus subsp. aureus HO 5096 0412]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAAGTAAAGAAATGGAACAAATTTCCGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT27697 WP_001251224.1 NC_017763:c1937275-1936676 [Staphylococcus aureus subsp. aureus HO 5096 0412]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT27697 NC_017763:c1937275-1936676 [Staphylococcus aureus subsp. aureus HO 5096 0412]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Antitoxin

• AlphaFold DB: W8U4V4